Anti-pan Keratin antibody [80] (ab8068)

We are not aware of any tests of rabbit samples with the pan-keratin antibody ab8068. The production and initial characterization of the antibody are described in the two 1984 papers at these links.


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900394/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900469/


I think the designation of the antibody as "pan-keratin" is based on the following comment from the Discussion section of the Jan 1984 paper, but we have not seen data from tests of purified keratins 3, 12, or others, or data from studies of cornea, so we can't say for sure that the antibody will be capable of detecting those keratins.


It is clear from our staining results on normal tissues and malignant epithelial tumors that Clone 80 is a very broadly reacting antibody directed against an antigenic determinant common to a number of cytokeratins present in almost all epithelial cells.

Thank you for contacting Abcam. I have looked through our catalogue to see if we have any prepared slides of human callus or keratinocytes. I have not been able to find anything that matches your requirements, but I have placed a link below that will show all of the prepared slides that we do have: www.abcam.com/index.html?pageconfig=catalog_byclass&intClassID=4186&strStartChar=A,B I also did a quick search of other companies to see if they had anything that matches your requirements but I could not find anything. Please let me know if there is anything else I can help with.  

Thank you for your question. We do not have any information about cross reactivity with pig. Please do not hesitate to contact us in case of any additional questions or remarks.

Thank you for getting back to me and for explaining a little bit more about your experiments. It may be worth applying antibodies which recognize pig albumin or serum (ab79960, ab50337, ab79957). Unfortunately, currently we do not have many antibodies specific for porcine proteins. The majority of our antibodies has been routinely tested in human, mouse and rat. You could use antibodies against mouse MHC (class I) to detect and distinguish mouse cells from porcine cells. We have different clonality antibodies against mouse MHC class I, please have a look and use our new search engine. You can also filter through the search results by different criteria (such as application, reactivity, clonality): https://www.abcam.com/ I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. This antibody has been tested in human, mouse, rat as the datasheet indicates, however no data available yet regarding porcine tissue. It does not necessary mean that ab8068 is unsuitable in this species but it has not been characterized yet for cross-reaction. Although, you have kindly provided some details, it would be much appreciated if you could give me more information about the staining (such as dilution/concentration with the primary antibody, blocking time and temperature, positive control etc) and complete the following form (attached as a word document). I could take a look at the protocol (if you wish) to see if any tips/recommendations can be made to improve the signal. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.