Anti-pan Keratin antibody [80] (ab8068)
![Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068-13506-anti-pan-keratin-antibody-80-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)")
Key features and details
- Mouse monoclonal [80] to pan Keratin
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-pan Keratin antibody [80]
See all pan Keratin primary antibodies -
Description
Mouse monoclonal [80] to pan Keratin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Callus cytokeratins isolated from fresh human skin tissue
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Positive control
- Human callus or keratinocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.07% Sodium azide
Constituent: 1% Fetal calf serum -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
80 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/20.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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| ICC/IF | (5) |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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Flow Cyt
1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Relevance
Cytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3848 Human
- Omim: 139350 Human
- SwissProt: P04264 Human
- Unigene: 80828 Human
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Alternative names
- CK1 antibody
- Cytokeratin 1 antibody
- EHK1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068-293227-anti-pan-keratin-antibody-80-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous AbreviewAb8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.
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![Western blot - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068-13505-anti-pan-keratin-antibody-80-western-blot.jpg)
Western blot - Anti-pan Keratin antibody [80] (ab8068)Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
![Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068-13504-anti-pan-keratin-antibody-80-flow-cytometry.jpg)
Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab8068 has been referenced in 26 publications.
- Cao J et al. Estrogen attenuates TGF-ß1-induced EMT in intrauterine adhesion by activating Wnt/ß-catenin signaling pathway. Braz J Med Biol Res 53:e9794 (2020). PubMed: 32638833
- Wang Y et al. S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway. Invest Ophthalmol Vis Sci 61:19 (2020). PubMed: 32926102
- Lytle NK et al. A Multiscale Map of the Stem Cell State in Pancreatic Adenocarcinoma. Cell 177:572-586.e22 (2019). PubMed: 30955884
- Hu J et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
- Chartoumpekis DV et al. Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice. JCI Insight 3:N/A (2018). PubMed: 29515034