- Datasheet
- Specific References (17)
- Protocols
Overview
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Product nameAnti-pan Keratin antibody [80]
See all pan Keratin primary antibodies -
DescriptionMouse monoclonal [80] to pan Keratin
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Callus cytokeratins isolated from fresh human skin tissue
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Positive control
- Human callus or keratinocytes
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.07% Sodium azide
Constituent: 1% Fetal calf serum -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone number80
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells. | |
| IHC-P | Use at an assay dependent concentration. For paraffin embedded tissue a TUF pretreatment is recommended. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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RelevanceCytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 3848 Human
- Omim: 139350 Human
- SwissProt: P04264 Human
- SwissProt: P04104 Mouse
- Unigene: 80828 Human
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Alternative names
- CK1 antibody
- Cytokeratin 1 antibody
- EHK1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068_review_12189.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous Abreviewab8068 staining human foreskin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0 in boiling water bath for 10 minutes, followed by cooling at room temperature for 30 minutes. The blocking was done using 1%BSA/5% normal donkey serum in PBS pH 7.4 for 2 hours at room temperature. The primary antibody, diluted 1/10 (PBS pH 7.4, 1%BSA, 0.1% sodium azide) and incubated with sample for 16 hours at 4°C. A HRP-conjugated donkey polyclonal to mouse Ig, diluted 1/1000 was used as the secondary.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/pan-Keratin-Primary-antibodies-ab8068-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/ab8068-293227-ab8068ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous AbreviewAb8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.
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![Western blot - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/pan-Keratin-Primary-antibodies-ab8068-3.jpg)
Western blot - Anti-pan Keratin antibody [80] (ab8068)Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
![Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/pan-Keratin-Primary-antibodies-ab8068-4.jpg)
Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)](https://www.abcam.com/ps/products/8/ab8068/Images/pan-Keratin-Primary-antibodies-ab8068-25.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)Image courtesy of an anonymous Abreview.ab8068 staining pan Keratin in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab8068 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-mouse polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.
Protocols
References
This product has been referenced in:
- Chartoumpekis DV et al. Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice. JCI Insight 3:N/A (2018). Read more (PubMed: 29515034) »
- Jiang Z et al. LincIN, a novel NF90-binding long non-coding RNA, is overexpressed in advanced breast tumors and involved in metastasis. Breast Cancer Res 19:62 (2017). Read more (PubMed: 28558830) »
Customer reviews and Q&As
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