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  1. Link

    pan-keratin-antibody-80-ab8068.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Cytokeratin
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Anti-pan Keratin antibody [80] (ab8068)

  • Datasheet
  • SDS
Reviews (11)Q&A (10)References (26)

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Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
  • Western blot - Anti-pan Keratin antibody [80] (ab8068)
  • Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)

Key features and details

  • Mouse monoclonal [80] to pan Keratin
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-pan Keratin antibody [80]
    See all pan Keratin primary antibodies
  • Description

    Mouse monoclonal [80] to pan Keratin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Callus cytokeratins isolated from fresh human skin tissue

  • Positive control

    • Human callus or keratinocytes
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.07% Sodium azide
    Constituent: 1% Fetal calf serum
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    80
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Cytokeratin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF (5)
Use a concentration of 1 µg/ml.
Notes
Flow Cyt
1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Relevance

    Cytokeratin is a family of basic and acidic proteins, present in dermal tissues. Each cytokeratin is formed by heterotetramers of different types of keratin, that span from 1 to 18. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3848 Human
    • Omim: 139350 Human
    • SwissProt: P04264 Human
    • Unigene: 80828 Human
    • Alternative names

      • CK1 antibody
      • Cytokeratin 1 antibody
      • EHK1 antibody
      • K1 antibody
      • Keratin 1 antibody
      • KRT1 antibody
      • KRT1A antibody
      • KRTA antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous Abreview

      Ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.

      See Abreview

    • Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Western blot - Anti-pan Keratin antibody [80] (ab8068)
      Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 65 kDa
      Observed band size: 65 kDa
      Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)
      Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (26)

    Publishing research using ab8068? Please let us know so that we can cite the reference in this datasheet.

    ab8068 has been referenced in 26 publications.

    • Cao J  et al. Estrogen attenuates TGF-ß1-induced EMT in intrauterine adhesion by activating Wnt/ß-catenin signaling pathway. Braz J Med Biol Res 53:e9794 (2020). PubMed: 32638833
    • Wang Y  et al. S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway. Invest Ophthalmol Vis Sci 61:19 (2020). PubMed: 32926102
    • Lytle NK  et al. A Multiscale Map of the Stem Cell State in Pancreatic Adenocarcinoma. Cell 177:572-586.e22 (2019). PubMed: 30955884
    • Hu J  et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
    • Chartoumpekis DV  et al. Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice. JCI Insight 3:N/A (2018). PubMed: 29515034
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 21 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocytes)
    Permeabilization
    No
    Specification
    Keratinocytes
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Epithelial cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Epithelial cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 22 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cervix)
    Permeabilization
    No
    Specification
    Cervix
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 08 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hair follicle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH=6
    Permeabilization
    No
    Specification
    Hair follicle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2020

    Immunocytochemistry abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Keratinocytes (HaCaT))
    Permeabilization
    No
    Specification
    Keratinocytes (HaCaT)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: PBS°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2020

    Question

    I was wondering whether the abcam 8068 pan Keratin antibody might also work for rabbit tissue. More specifically we would like to use this antibody to stain the cornea which is mainly positive for Keratin 3 and 12 (also for CK 14 and 15). Would this Ab be able to detect (Cyto)Keratin 3 and 12?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2018

    Answer

    We are not aware of any tests of rabbit samples with the pan-keratin antibody ab8068. The production and initial characterization of the antibody are described in the two 1984 papers at these links.


    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900394/

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1900469/


    I think the designation of the antibody as "pan-keratin" is based on the following comment from the Discussion section of the Jan 1984 paper, but we have not seen data from tests of purified keratins 3, 12, or others, or data from studies of cornea, so we can't say for sure that the antibody will be capable of detecting those keratins.


    It is clear from our staining results on normal tissues and malignant epithelial tumors that Clone 80 is a very broadly reacting antibody directed against an antigenic determinant common to a number of cytokeratins present in almost all epithelial cells.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin (psoriasis))
    Permeabilization
    No
    Specification
    Skin (psoriasis)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Herr Dr. Christian Ostalecki

    Verified customer

    Submitted May 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell line)
    Specification
    human glioblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Keratin antibody [80]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (glioblastoma cell line C6)
    Specification
    glioblastoma cell line C6
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2012

    1-10 of 21 Abreviews or Q&A

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