Anti-pan Lamin antibody [X67, X167, X233] (ab20740)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-pan Lamin antibody [X67, X167, X233] -
Description
Mouse monoclonal [X67, X167, X233] to pan Lamin -
Host species
Mouse -
Specificity
Lamins do not appear to be universally distributed among different cell and tissue types. ab20740 recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C). Other cell/tissue types have not been tested. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout -
Immunogen
cell preparation: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
X67, X167, X233 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab20740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
ICC/IF |
Target
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Relevance
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4000 Human
- Entrez Gene: 4001 Human
- Entrez Gene: 84823 Human
- SwissProt: P02545 Human
- SwissProt: P20700 Human
- SwissProt: Q03252 Human
- Unigene: 538286 Human
- Unigene: 594444 Human
see all -
Alternative names
- ADLD antibody
- CMD1A antibody
- CMT2B1 antibody
see all
Images
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ab20740 at a 1/50 dilution staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG.
The antibody was found to stain equally well when used at a dilution of 1/250.
This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.
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Immunocytochemistry/ Immunofluorescence - Anti-pan Lamin antibody [X67, X167, X233] (ab20740)Image from Mackay DR et al, J Cell Biol. 2010 Nov 29;191(5):923-31. Epub 2010 Nov 22, Fig 1. DOI 10.1083/jcb.201007124ab20740 staining pan Lamin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Left image shows pan Lamin.Right image shows overlay of pan lamin (green), tubulin (red) and DNA (blue).
Datasheets and documents
References
This product has been referenced in:
- Shibano T et al. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PLoS One 10:e0127271 (2015). Read more (PubMed: 25946333) »
- Azevedo AR et al. Molecular Cytogenetics of Human Single Pronucleated Zygotes. Reprod Sci N/A:N/A (2014). Read more (PubMed: 24717739) »