Anti-pan Lamin antibody [X67, X167, X233] (ab20740)

Reviews (2)Q&A (3)References (5)

Key features and details

  • Mouse monoclonal [X67, X167, X233] to pan Lamin
  • Reacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout
  • Isotype: IgG1

Overview

  • Product name

    Anti-pan Lamin antibody [X67, X167, X233]

  • Description

    Mouse monoclonal [X67, X167, X233] to pan Lamin

  • Host species

    Mouse

  • Specificity

    Lamins do not appear to be universally distributed among different cell and tissue types. ab20740 recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C). Other cell/tissue types have not been tested.

  • Species reactivity

    Reacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout

  • Immunogen

    Tissue, cells or virus corresponding to Xenopus laevis pan Lamin. Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Target

  • Relevance

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • ADLD antibody
      • CMD1A antibody
      • CMT2B1 antibody
      • Lamin A/C antibody
      • Lamin B1 antibody
      • Lamin B2 antibody
      • LMN2 antibody
      • LMNA antibody
      • LMNB1 antibody
      • LMNB2 antibody
      see all

    References (5)

    Publishing research using ab20740? Please let us know so that we can cite the reference in this datasheet.

    ab20740 has been referenced in 5 publications.

    • Lionetti MC  et al. Chromatin and Cytoskeletal Tethering Determine Nuclear Morphology in Progerin-Expressing Cells. Biophys J 118:2319-2332 (2020). PubMed: 32320674
    • Shibano T  et al. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PLoS One 10:e0127271 (2015). PubMed: 25946333
    • Azevedo AR  et al. Molecular Cytogenetics of Human Single Pronucleated Zygotes. Reprod Sci N/A:N/A (2014). PubMed: 24717739
    • Mackay DR  et al. Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint. J Cell Biol 191:923-31 (2010). ICC/IF ; Human . PubMed: 21098116
    • Nilsen T  et al. A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. J Biol Chem 282:26245-56 (2007). PubMed: 17616529

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Lamin antibody [X67, X167, X233]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Schmidtea mediterranea Tissue sections (whole animal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 95 degree 15min citrate buffer pH6.0
    Permeabilization
    Yes - PBST 15min
    Specification
    whole animal
    Blocking step
    Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 28°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Lamin antibody [X67, X167, X233]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2 OS)
    Specification
    U2 OS
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5
    Read More

    Dr. Chi W Tang

    Verified customer

    Submitted Apr 06 2006

    Question

    Will this antibody cross-react with Lamin C? What samples were used to test this antibody in Western blotting?

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    Answer

    Thank you for your enquiry and patience. This product is a mixture of 3 clones [X67, X167, X233] and two of the clones, X67 and X167, do react with Lamin C. I'm not able to obtain information at this time on exactly what samples were used to test this antibody. Please contact us again if you have any additional questions.

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    Question

    BATCH NUMBER 185267 ORDER NUMBER seebelow DESCRIPTION OF THE PROBLEM Not correct staining (non-specific staining throughout nucleus and cytoplasm) SAMPLE BJ fibroblasts, Hela cells (cell lines) PRIMARY ANTIBODY Abcam Lamin A antibody [X67, X167, X233] (ab20740) Have tried as suggested on sheet - 16hrs, 1/50 dilution at 4 degrees diluted in 1% BSA Have also tried undiluted, 1/2, and 1/25 with no success washed with PBS POSITIVE AND NEGATIVE CONTROLS USED Tested cells with lamin Ab I know works, and cells were fine. Tried the secondary with another mouse Ab and it worked as expected. Tried Lamin A antibody [X67, X167, X233] with another secondary and it didn't work. ANTIBODY STORAGE CONDITIONS 4 degrees celcius FIXATION OF SAMPLE 4% Formaldehyde for 20 minutes PERMEABILIZATION STEP 0.2% Triton X-100 for 20 minutes BLOCKING CONDITIONS 1% BSA, 20 minutes SECONDARY ANTIBODY Abcam goat anti-mouse IgG at 1:200 diluted in 1% BSA HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? primary and secondary concentration, secondary ab (used different anti-mouse) ADDITIONAL NOTES purchuse order number wouldn't fit. It is 0300027711

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    Answer

    Thank you for contacting me regarding the problems that you have experienced using this Ab. Where an Ab is giving non specific staining I would suggest decreasing the concentration at which the primary Ab is used at from 1/50 to 1/100 rather than using the Ab at the higher concentration of ½ and undiluted, the reviewer mentions that using a concentration of 1/250 has been observed to give the same results. I would also advise that you use saponin as a permeabalization reagent (5-10ug/ml) rather than Triton X-100. Triton-X 100 will destroy cellular membranes since the target is located in the nuclear membrane this may be what is resulting in non specific binding, releasing antigen into the cytoplasm. Saponin is a detergent that permeabalizes the cell membrane of eukaryotic cell membranes by interacting with cholesterol and is more gentle than Triton-X. Where membranes are not over treated with saponin they have the ability to reform. In addition to this the percentage of blocking reagent may be increased to 5% BSA this will also act to reduce the observed background staining. Please do not hesitate to get back in touch with me should you still experience problems.

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    Question

    Do you have information about the epitope recognized by this antibody.

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    Answer

    Thank you for your enquiry. This product is a mixture of 3 monoclonal antibodies, Clones X67, X167, and X223. The specific epitope locations have not been determined. The reactivity to lamin isotypes in different species for each clone is listed on the datasheet. Please contact us again if you require further assistance.

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