Key features and details
- Mouse monoclonal [X67, X167, X233] to pan Lamin
- Suitable for: ICC/IF
- Reacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout
- Isotype: IgG1
Product nameAnti-pan Lamin antibody [X67, X167, X233]
DescriptionMouse monoclonal [X67, X167, X233] to pan Lamin
SpecificityLamins do not appear to be universally distributed among different cell and tissue types. ab20740 recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C). Other cell/tissue types have not been tested.
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout
cell preparation: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Clone numberX67, X167, X233
Light chain typeunknown
Our Abpromise guarantee covers the use of ab20740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
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ab20740 at a 1/50 dilution staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG.
The antibody was found to stain equally well when used at a dilution of 1/250.
This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.
ab20740 staining pan Lamin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Left image shows pan Lamin.Right image shows overlay of pan lamin (green), tubulin (red) and DNA (blue).
ab20740 has been referenced in 4 publications.
- Shibano T et al. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PLoS One 10:e0127271 (2015). PubMed: 25946333
- Azevedo AR et al. Molecular Cytogenetics of Human Single Pronucleated Zygotes. Reprod Sci N/A:N/A (2014). PubMed: 24717739
- Mackay DR et al. Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint. J Cell Biol 191:923-31 (2010). ICC/IF ; Human . PubMed: 21098116
- Nilsen T et al. A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. J Biol Chem 282:26245-56 (2007). PubMed: 17616529