Overview

  • Product name
    Anti-pan Macrophage antibody [RM-1]
  • Description
    Mouse monoclonal [RM-1] to pan Macrophage
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab15615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: 1/100. 2% paraformaldehyde-lysine / sodium periodate or acetone fixation.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
    • Cellular localization
      Cell Membrane

    References

    This product has been referenced in:
    • Kasper M  et al. Heterogeneous Dolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem 95:1-11 (1993). Read more (PubMed: 8279229) »
    • Izumi S  et al. Ontogenetic development of synovial A cells in fetal and neonatal rat knee joints. Cell Tissue Res 262:1-8 (1990). Read more (PubMed: 2257604) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. This clone recognizes a surface antigen of unknown funtion on monocytes, marcophages, interdigitating cells and their in vitro equivalents. The antibody has been tested for application in FACS and IHC-frozen sections and shown to cross-react with rat. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. For more information regarding this clone I suggest referring to the following publications: Kasper M et al. Heterogeneous Dolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem 95:1-11 (1993). PubMed: 8279229 Izumi S et al. Ontogenetic development of synovial A cells in fetal and neonatal rat knee joints. Cell Tissue Res 262:1-8 (1990). PubMed: 2257604 Kato T et al. Chemically induced transplantable malignant fibrous histiocytoma of the rat. Analyses with immunohistochemistry, immunoelectron microscopy and [3H]thymidine autoradiography. Lab Invest 62:635-45 (1990). PubMed: 2160564 Takeya M et al. Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study. J Histochem Cytochem 37:635-41 (1989). PubMed: 2649558

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up