Recombinant
RabMAb

Recombinant Anti-pan-myc antibody [EPR18863] (ab195207)

Overview

  • Product name

    Anti-pan-myc antibody [EPR18863]
    See all pan-myc primary antibodies
  • Description

    Rabbit monoclonal [EPR18863] to pan-myc
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human pan-myc aa 1-100. The exact sequence is proprietary. P01106=Myc proto-oncogene protein. P04198=N-myc proto-oncogene protein. P12524=Protein L-Myc.
    Database link: P01106

  • Positive control

    • WB: HEK-293 whole cell lysate transfected with n-Myc with GFP-tag; HEK-293 whole cell lysate transfected with c-Myc; HEK-293 whole cell lysate transfected with L-Myc with GFP-tag; RAW 264.7, K562, HeLa and HEK-293 whole cell lysates. ICC/IF: HeLa and RAW 264.7 cells. FC: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18863
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab195207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 49, 50, 40 kDa).
Flow Cyt 1/60.

Target

  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe37 antibody
    • bHLHe38 antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • Class E basic helix-loop-helix protein 38 antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • L Myc antibody
    • l-myc-1 proto-oncogene antibody
    • LMYC antibody
    • MODED antibody
    • MRTL antibody
    • MYC antibody
    • Myc proto oncogene protein antibody
    • Myc related gene from lung cancer antibody
    • Myc related translation/localization regulatory factor antibody
    • MYCC antibody
    • MYCL antibody
    • MYCL1 antibody
    • MYCN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • Protein L Myc antibody
    • Protein L-Myc-1 antibody
    • Proto oncogene c Myc antibody
    • Transcription factor p64 antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc avian myelocytomatosis viral oncogene lung carcinoma derived homolog antibody
    • v myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc myelocytomatosis viral oncogene homolog 1 lung carcinoma derived antibody
    • v myc myelocytomatosis viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/5000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 whole cell lysate transfected with n-Myc with GFP-tag

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 83 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on RAW 264.7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow cytometry analysis of HeLa cells labelling pan-myc (red) with purified ab195207 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 whole cell lysate transfected with c-Myc

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 whole cell lysate transfected with L-Myc with GFP-tag

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 83, 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight for the mouse species is slightly higher than human Myc, this could be due to different degree of glycosylation.

  • Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution + K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49, 50, 40 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab195207 has not yet been referenced specifically in any publications.

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