Recombinant Anti-pan-myc antibody [EPR18863] (ab195207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18863] to pan-myc
- Suitable for: ICC/IF, WB, Flow Cyt (Intra), ChIC/CUT&RUN-seq
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-pan-myc antibody [EPR18863]
See all pan-myc primary antibodies -
Description
Rabbit monoclonal [EPR18863] to pan-myc -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra), ChIC/CUT&RUN-seqmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 whole cell lysate transfected with n-Myc with GFP-tag; HEK-293 whole cell lysate transfected with c-Myc; HEK-293 whole cell lysate transfected with L-Myc with GFP-tag; RAW 264.7, K562, HeLa and HEK-293 whole cell lysates. ICC/IF: HeLa and RAW 264.7 cells. Flow Cyt (intra): HeLa cells ChIC/CUT&RUN-Seq: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18863 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/250.
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WB |
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 49, 50, 40 kDa).
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Flow Cyt (Intra) |
1/60.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5µg |
Notes |
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ICC/IF
1/250. |
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 49, 50, 40 kDa). |
Flow Cyt (Intra)
1/60. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5µg |
Target
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Cellular localization
Nuclear -
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 4610 Human
- Entrez Gene: 4613 Human
- Entrez Gene: 16918 Mouse
- Entrez Gene: 17869 Mouse
- Entrez Gene: 18109 Mouse
- Omim: 164840 Human
- Omim: 164850 Human
see all -
Alternative names
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe37 antibody
- bHLHe38 antibody
see all
Images
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5µg of ab195207 [EPR18863]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/5000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with n-Myc with GFP-tag
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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Intracellular Flow Cytometry analysis of HeLa cells labelling pan-myc (red) with purified ab195207 at dilution of 1/60. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with c-Myc
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with L-Myc with GFP-tag
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 83, 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight for the mouse species is slightly higher than human Myc, this could be due to different degree of glycosylation.
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Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution + K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab195207 has been referenced in 1 publication.
- Lee PC et al. Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinoma. J Clin Invest 132:N/A (2022). PubMed: 35775490