Key features and details
- Rabbit polyclonal to Pan-SAPAP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Pan-SAPAP antibody
DescriptionRabbit polyclonal to Pan-SAPAP
Please note that the immunogen that generated ab67224 shares some sequence with other SAPAP/DLGAP proteins, including SAPAP1, SAPAP2, and SAPAP4, and preliminary data suggests this antibody may not be specific for SAPAP3.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Pan-SAPAP.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceSAPAP3, (DLGAP3) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP3 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). Both SAPAP3 protein and mRNA are targeted to dendrites, whereas SAPAP1, -2, and -4 mRNAs are detected mainly in cell bodies. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. At least two isoforms are known to exist.
Cellular localizationCell Membrane
- DLGAP3 antibody
- DAP3 antibody
- Discs, large (Drosophila) homolog associated protein 3 antibody
Immunofluorescence of Pan-SAPAP in Human brain tissue with ab67224 at 20 ug/mL.
Lane 1 : Anti-Pan-SAPAP antibody (ab67224) at 1 µg/ml
Lane 2 : Anti-Pan-SAPAP antibody (ab67224) at 2 µg/ml
All lanes : rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
ab67224 at 2.5µg/ml staining of Pan-SAPAP in human brain tissue using IHC-P.
ab67224 has not yet been referenced specifically in any publications.