Anti-pan Synuclein antibody (ab53726)
Key features and details
- Rabbit polyclonal to pan Synuclein
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-pan Synuclein antibody
See all pan Synuclein primary antibodies -
Description
Rabbit polyclonal to pan Synuclein -
Host species
Rabbit -
Specificity
Detects endogenous levels of total Synuclein protein. This antibody recognises all forms of synuclein (alpha, beta and gamma). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human pan Synuclein aa 15-64. The exact sequence is proprietary. Synthetic peptide derived from human synuclein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development. -
Cellular localization
Neuronal cytosol and presynaptic terminals -
Database links
- Entrez Gene: 6620 Human
- Entrez Gene: 6622 Human
- Entrez Gene: 6623 Human
- Entrez Gene: 104069 Mouse
- Entrez Gene: 20617 Mouse
- Entrez Gene: 20618 Mouse
- Entrez Gene: 113893 Rat
- Entrez Gene: 29219 Rat
see all -
Alternative names
- BCSG1 antibody
- beta synuclein antibody
- Breast cancer specific gene 1 protein antibody
see all
Images
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All lanes : Anti-pan Synuclein antibody (ab53726) at 1/500 dilution
Lane 1 : rat heart lysate
Lane 2 : rat heart lysate with immunizing peptide
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size: 14 kDa
Observed band size: 14 kDa -
Ab53726 at 1/50 dilution, staining paraffin embedded human brain in the presence (right) or absence (left) of blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab53726 has been referenced in 5 publications.
- Soll LG et al. a-Synuclein-112 Impairs Synaptic Vesicle Recycling Consistent With Its Enhanced Membrane Binding Properties. Front Cell Dev Biol 8:405 (2020). PubMed: 32548120
- McGlinchey RP et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
- Medeiros AT et al. a-Synuclein Dimers Impair Vesicle Fission during Clathrin-Mediated Synaptic Vesicle Recycling. Front Cell Neurosci 11:388 (2017). PubMed: 29321725
- Busch DJ et al. Acute increase of a-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation. Mol Biol Cell 25:3926-41 (2014). PubMed: 25273557
- Lau BY et al. Cyclic AMP promotes axon regeneration, lesion repair and neuronal survival in lampreys after spinal cord injury. Exp Neurol 250C:31-42 (2013). WB, IHC-P . PubMed: 24041988