Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1058Y] to Pan Trk (PE)
- Suitable for: Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-Pan Trk antibody [EP1058Y] (PE)
See all Pan Trk primary antibodies
DescriptionRabbit monoclonal [EP1058Y] to Pan Trk (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThis antibody detects both phosphorylated and unphosphorylated TrkA.
Tested applicationsSuitable for: Flow Cytmore details
Predicted to work with: Mouse, Rat, Human
within Human Pan Trk aa 750 to the C-terminus. The exact sequence is proprietary. (A synthetic peptide corresponding to residues surrounding tyrosine 791 of human Pan Trk).
Database link: P04629
- Flow Cyt: U87MG cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Rat brain normal tissue lysate - membrane extract (ab29473)
- Mouse brain tissue lysate - total protein (ab30151)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
Our Abpromise guarantee covers the use of ab209443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
RelevanceFamily of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
Overlay histogram showing U87MG cells stained with ab209443 (red line). The cells were fixed with 4% formaldehyde (10 min)and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209443, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
ab209443 has not yet been referenced specifically in any publications.