Anti-Pan Trk antibody [EPR17341] (ab181560)
Rabbit recombinant monoclonal Pan Trk antibody [EPR17341]. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-Pan Trk antibody [EPR17341]
See all Pan Trk primary antibodies -
DescriptionRabbit monoclonal [EPR17341] to Pan Trk
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Pan Trk aa 800 to the C-terminus. The exact sequence is proprietary.
Database link: Q16620 -
Positive control
- WB: Human fetal brain and cerebellum lysates, Mouse and Rat brain lysates. IHC-P: Human astrocytoma and cerebral cortex tissue, Mouse cerebral cortex tissue, Rat cerebral cortex tissue. ICC/IF: Neuro-2a cells.
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General notes
This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay). For more information, please visit the following webpage.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR17341
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab181560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/10000. Detects a band of approximately 30,140 kDa (predicted molecular weight: 92 kDa). |
Target
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RelevanceFamily of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
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Database links
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all -
Alternative names
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232330-1815604.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Astrocytoma cells show strong cytoplasmic staining. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232339-18156011.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Pan Trk with ab181560 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image showing cytoplasmic staining on Neuro-2a cells is shown. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab181560 at 1/250 dilution followed by ab150120 (Goat anti mouse IgG (Alexa Fluor® 594)) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Goat anti rabbit IgG (Alexa Fluor® 488)) at 1/400 dilution. -
![Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232327-181560.JPG)
Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)All lanes : Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous systems,
human fetal heart and human fetal spleen are used as negative controls.
The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232331-1815605.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of human cerebral cortex. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232334-1815606.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on normal Human liver. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
![Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232472-ab-181-wb-3-amend.jpg)
Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)All lanes : Extraction Buffer (ab191560) at 1/10000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat kidney lysates
Lane 6 : Rat spleen lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous system. Mouse kidney, mouse spleen, rat kidney and rat spleen are used as negative control.
The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232335-1815607.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232336-1815608.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on Mouse liver. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232337-1815609.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of Rat cerebral cortex. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232338-18156010.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on Rat liver. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
![Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)](https://www.abcam.com/ps/products/181/ab181560/Images/ab181560-232328-1815602.JPG)
Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution + Human cerebellum lysates at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
Protocols
References
This product has been referenced in:
- Ziegler DS et al. Brief Report: Potent clinical and radiological response to larotrectinib in TRK fusion-driven high-grade glioma. Br J Cancer 119:693-696 (2018). IHC-P ; Human . Read more (PubMed: 30220707) »
- Chiang S et al. NTRK Fusions Define a Novel Uterine Sarcoma Subtype With Features of Fibrosarcoma. Am J Surg Pathol 42:791-798 (2018). Read more (PubMed: 29553955) »
