Recombinant
RabMAb

Recombinant Anti-Pan Trk antibody [EPR17341] (ab181560)

Rabbit recombinant monoclonal Pan Trk antibody [EPR17341]. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).

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Overview

  • Product name

    Anti-Pan Trk antibody [EPR17341]
    See all Pan Trk primary antibodies

  • Description

    Rabbit monoclonal [EPR17341] to Pan Trk

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken

  • Immunogen

    Synthetic peptide within Human Pan Trk aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16620

  • Positive control

    • WB: Human fetal brain and cerebellum lysates, Mouse and Rat brain lysates. IHC-P: Human astrocytoma and cerebral cortex tissue, Mouse cerebral cortex tissue, Rat cerebral cortex tissue. ICC/IF: Neuro-2a cells.

  • General notes

    This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay). 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab181560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000. Detects a band of approximately 30,140 kDa (predicted molecular weight: 92 kDa).

Target

  • Relevance

    Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.

  • Database links

    see all

  • Alternative names

    • BDNF/NT-3 growth factors receptor antibody
    • EML4 antibody
    • ETV6 antibody
    • gp140trk antibody
    • GP145-TrkB antibody
    • GP145-TrkC antibody
    • High affinity nerve growth factor receptor antibody
    • LMNA antibody
    • mammary analogue secretory carcinoma antibody
    • MTC antibody
    • neuroendocrine tumor antibody
    • neurotrophic antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophic tyrosine kinase receptor type 3 antibody
    • NT-3 growth factor receptor antibody
    • NTRK antibody
    • NTRK1 antibody
    • NTRK2 antibody
    • NTRK3 antibody
    • p140-TrkA antibody
    • TPM3 antibody
    • TRK antibody
    • TRK fusion antibody
    • Trk-A antibody
    • Trk-B antibody
    • Trk-C antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • TRKA antibody
    • trka+b+c antibody
    • trka/b/c antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKC antibody
    • TrkC tyrosine kinase antibody
    • tropomyosin receptor kinase antibody
    • Tropomyosin-related kinase A antibody
    • Tropomyosin-related kinase B antibody
    • Tropomyosin-related kinase C antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    • tyrosine kinase receptor B antibody
    • tyrosine kinase receptor C antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural mix culture cells labelling Pan Trk with ab181560 at 1:100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1:1000 dilution (Green). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1:200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Pan Trk with ab181560 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image showing cytoplasmic staining on Neuro-2a cells is shown. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows;
    1. ab181560 at 1/250 dilution followed by ab150120 (Goat anti mouse IgG (Alexa Fluor® 594)) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Goat anti rabbit IgG (Alexa Fluor® 488)) at 1/400 dilution.

  • Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    All lanes : Extraction Buffer (ab191560) at 1/10000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat kidney lysates
    Lane 6 : Rat spleen lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa



    TrkB is abundantly expressed in the central and peripheral nervous system. Mouse kidney, mouse spleen, rat kidney and rat spleen are used as negative control.
    The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Astrocytoma cells show strong cytoplasmic staining. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    All lanes : Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human fetal spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 92 kDa



    TrkB is abundantly expressed in the central and peripheral nervous systems,

    human fetal heart and human fetal spleen are used as negative controls.

    The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of human cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Western blot - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution + Human cerebellum lysates at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 92 kDa



    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of mouse cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of Rat cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on normal Human liver. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on Mouse liver. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on Rat liver. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

References

This product has been referenced in:

  • Davis JL  et al. Expanding the Spectrum of Pediatric NTRK-rearranged Mesenchymal Tumors. Am J Surg Pathol 43:435-445 (2019). Read more (PubMed: 30585824) »
  • Solomon JP  et al. NTRK fusion detection across multiple assays and 33,997 cases: diagnostic implications and pitfalls. Mod Pathol N/A:N/A (2019). IHC-P ; Human . Read more (PubMed: 31375766) »
See all 21 Publications for this product

Publishing research using ab181560? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-TrkA + TrkB + TrkC antibody [EPR17341]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y Neuroblastoma Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Treatment
10 µM All trans retionic Acid (ATRA) for 5 days
Specification
SH-SY5Y Neuroblastoma Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Dr. Lars Riecken

Verified customer

Submitted Oct 23 2018

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