Product nameAnti-Pan Trk antibody [EPR17341] (HRP)
See all Pan Trk primary antibodies
DescriptionRabbit monoclonal [EPR17341] to Pan Trk (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide within Human Pan Trk aa 800 to the C-terminus. The exact sequence is proprietary.
Database link: Q16620
- WB: Mouse Brain and Rat Brain tissue lysates
This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 119 kDa (predicted molecular weight: 92 kDa).|
RelevanceFamily of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
All lanes : Anti-Pan Trk antibody [EPR17341] (HRP) (ab209465) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 119 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209465 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209465 has not yet been referenced specifically in any publications.