Recombinant
RabMAb

Recombinant Anti-Pan Trk antibody [EPR17341] (HRP) (ab209465)

Overview

  • Product name

    Anti-Pan Trk antibody [EPR17341] (HRP)
    See all Pan Trk primary antibodies
  • Description

    Rabbit monoclonal [EPR17341] to Pan Trk (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human Pan Trk aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16620

  • Positive control

    • WB: Mouse Brain and Rat Brain tissue lysates
  • General notes

    This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay).

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17341
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab209465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 119 kDa (predicted molecular weight: 92 kDa).

Target

  • Relevance

    Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
  • Database links

  • Alternative names

    • BDNF/NT-3 growth factors receptor antibody
    • EML4 antibody
    • ETV6 antibody
    • gp140trk antibody
    • GP145-TrkB antibody
    • GP145-TrkC antibody
    • High affinity nerve growth factor receptor antibody
    • LMNA antibody
    • mammary analogue secretory carcinoma antibody
    • MTC antibody
    • neuroendocrine tumor antibody
    • neurotrophic antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophic tyrosine kinase receptor type 3 antibody
    • NT-3 growth factor receptor antibody
    • NTRK antibody
    • NTRK1 antibody
    • NTRK2 antibody
    • NTRK3 antibody
    • p140-TrkA antibody
    • TPM3 antibody
    • TRK antibody
    • TRK fusion antibody
    • Trk-A antibody
    • Trk-B antibody
    • Trk-C antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • TRKA antibody
    • trka+b+c antibody
    • trka/b/c antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKC antibody
    • TrkC tyrosine kinase antibody
    • tropomyosin receptor kinase antibody
    • Tropomyosin-related kinase A antibody
    • Tropomyosin-related kinase B antibody
    • Tropomyosin-related kinase C antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    • tyrosine kinase receptor B antibody
    • tyrosine kinase receptor C antibody
    see all

Images

  • All lanes : Anti-Pan Trk antibody [EPR17341] (HRP) (ab209465) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 119 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209465 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209465 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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