Overview

  • Product name

    Anti-Pan Trk antibody [EPR18413]
    See all Pan Trk primary antibodies
  • Description

    Rabbit monoclonal [EPR18413] to Pan Trk
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pan Trk aa 800 to the C-terminus (phospho Y820) (Cysteine residue). The exact sequence is proprietary. The original immunogen was phospho peptide but the antibody failed to specifically recognize the phospho site, recognizing phospho and nonphospho protein equally.
    Database link: Q16288-3

  • Positive control

    • WB: Human, mouse and rat brain lysates; SH-SY5Y whole cell lysate; HEK-293 whole cell lysate transfected with TrkA, TrkB and TrkC, respectively. IP: Mouse brain whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
WB 1/1000. Detects a band of approximately 140,30 kDa (predicted molecular weight: 94 kDa).

Target

  • Relevance

    Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
  • Database links

  • Alternative names

    • BDNF/NT-3 growth factors receptor antibody
    • EML4 antibody
    • ETV6 antibody
    • gp140trk antibody
    • GP145-TrkB antibody
    • GP145-TrkC antibody
    • High affinity nerve growth factor receptor antibody
    • LMNA antibody
    • mammary analogue secretory carcinoma antibody
    • MTC antibody
    • neuroendocrine tumor antibody
    • neurotrophic antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophic tyrosine kinase receptor type 3 antibody
    • NT-3 growth factor receptor antibody
    • NTRK antibody
    • NTRK1 antibody
    • NTRK2 antibody
    • NTRK3 antibody
    • p140-TrkA antibody
    • TPM3 antibody
    • TRK antibody
    • TRK fusion antibody
    • Trk-A antibody
    • Trk-B antibody
    • Trk-C antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • TRKA antibody
    • trka+b+c antibody
    • trka/b/c antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKC antibody
    • TrkC tyrosine kinase antibody
    • tropomyosin receptor kinase antibody
    • Tropomyosin-related kinase A antibody
    • Tropomyosin-related kinase B antibody
    • Tropomyosin-related kinase C antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    • tyrosine kinase receptor B antibody
    • tyrosine kinase receptor C antibody
    see all

Images

  • All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution

    Lanes 1 & 3 & 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa



     

    Exposure times: Lane 1-2: 1 minute; Lane 3-6: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10 µg (Input).

    Lane 2: ab189903 IP in mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.

    Exposure time: 5 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

References

ab189903 has not yet been referenced specifically in any publications.

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