Overview

  • Product name

  • Description

    Rabbit polyclonal to Pan2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Pan2 aa 1-50. The exact sequence is proprietary.
    Database link: Q504Q3

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 135 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein.
  • Sequence similarities

    Belongs to the peptidase C19 family. PAN2 subfamily.
    Contains 1 exonuclease domain.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPan2 antibody
    • Inactive ubiquitin carboxyl terminal hydrolase 52 antibody
    • Inactive ubiquitin carboxyl-terminal hydrolase 52 antibody
    • KIAA0710 antibody
    • PAB dependent poly(A) specific ribonuclease subunit 2 antibody
    • PAB-dependent poly(A)-specific ribonuclease subunit 2 antibody
    • PABP dependent poly(A) nuclease 2 antibody
    • PAN 2 antibody
    • PAN2 antibody
    • PAN2 homolog PABP1 dependent poly A specific ribonuclease subunit antibody
    • PAN2 poly(A) specific ribonuclease subunit homolog antibody
    • PAN2 polyA specific ribonuclease subunit homolog antibody
    • PAN2_HUMAN antibody
    • Ubiquitin specific peptidase 52 antibody
    • Ubiquitin specific protease 52 antibody
    • USP 52 antibody
    • USP52 antibody
    see all

Images

  • All lanes : Anti-Pan2 antibody (ab241505) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 135 kDa


    Exposure time: 3 minutes


    Prepared using NETN lysis buffer.

  • Pan2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab241505 used for IP at 6 µg per reaction. For WB 1 µg/ml.

    Lane 1: ab241505 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEWK-293T whole cell lysate.

    Chemiluminescence detection: 3 minutes.

References

ab241505 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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