Key features and details
- Rabbit polyclonal to Pan2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Pan2 antibody
See all Pan2 primary antibodies
DescriptionRabbit polyclonal to Pan2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Pan2 aa 1-50. The exact sequence is proprietary.
Database link: Q504Q3
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to Pan2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 135 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionFunctions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein.
Sequence similaritiesBelongs to the peptidase C19 family. PAN2 subfamily.
Contains 1 exonuclease domain.
Cellular localizationCytoplasm. Nucleus. Shuttles between nucleus and cytoplasm.
- Information by UniProt
- hPan2 antibody
- Inactive ubiquitin carboxyl terminal hydrolase 52 antibody
- Inactive ubiquitin carboxyl-terminal hydrolase 52 antibody
All lanes : Anti-Pan2 antibody (ab241505) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 135 kDa
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
Pan2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241505 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab241505 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEWK-293T whole cell lysate.
Chemiluminescence detection: 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241505 has not yet been referenced specifically in any publications.