Overview

  • Product name

    Anti-Pan3 antibody
  • Description

    Rabbit polyclonal to Pan3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Pan3 aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102602)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab88642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).

Target

  • Function

    Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPan3 antibody
    • PAB dependent poly(A) specific ribonuclease subunit 3 antibody
    • PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
    • PAB1P dependent poly(A) nuclease antibody
    • PABP dependent poly(A) nuclease 3 antibody
    • PABP1 dependent poly A specific ribonuclease subunit PAN3 antibody
    • Pan 3 antibody
    • PAN deadenylation complex subunit 3 antibody
    • PAN3 antibody
    • PAN3 poly(A) specific ribonuclease subunit antibody
    • PAN3 poly(A) specific ribonuclease subunit homolog antibody
    • PAN3 polyA specific ribonuclease subunit homolog antibody
    • PAN3_HUMAN antibody
    see all

Images

  • All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma cell line N2A)
Loading amount
30 µg
Specification
Mouse Neuroblastoma cell line N2A
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 23 2012

Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 13 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 15 2011

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