Product nameAnti-Pancreatic alpha amylase antibody
See all Pancreatic alpha amylase primary antibodies
DescriptionRabbit polyclonal to Pancreatic alpha amylase
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
Full length native protein (purified) corresponding to Human Pancreatic alpha amylase.
- IHC-P: Human normal pancreas tissue. IHC-Fr: Mouse pancreas tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab21156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/8000. Predicted molecular weight: 57 kDa. Strong reaction to 0.15ug and 0.3ug antigen detected. Predicted molecular weight: 57 kDa. Dilution optimised using Chromogenic detection.|
|IHC-P||1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevancePancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).
Cellular localizationSecreted protein; extracellular space.
- 1 4 alpha D glucan glucanohydrolase antibody
- 1,4 alpha D glucan glucanohydrolase 2B antibody
- Alpha amylase 2B antibody
IHC image of Pancreatic Amylase staining in Human pancreas normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21156, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab21156 staining Pancreatic alpha amylase in mouse pancreas tissue in immunohistochemical analysis (Frozen sections).
Tissue was blocked with 5% BSA for 30 minutes at 21°C. Samples were incubated with primary antibody for 1 hour at 21°C at 1/800 dilution. Renaissance background reducing diluent was used. An Alexa Fluor488-conjugated goat anti-rabbit IgG monoclonal (H+L) was used as secondary antibody at 1/200 dilution.
This product has been referenced in:
- Shiokawa M et al. Pathogenicity of IgG in patients with IgG4-related disease. Gut N/A:N/A (2016). Human . Read more (PubMed: 26964842) »
- Khoriaty R et al. Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep 6:27802 (2016). IHC . Read more (PubMed: 27297878) »