Overview

  • Product name
    Anti-Pancreatic alpha amylase antibody
    See all Pancreatic alpha amylase primary antibodies
  • Description
    Rabbit polyclonal to Pancreatic alpha amylase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Pancreatic alpha amylase.

  • Positive control
    • IHC-P: Human normal pancreas tissue. IHC-Fr: Mouse pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab21156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Predicted molecular weight: 57 kDa. Strong reaction to 0.15ug and 0.3ug antigen detected. Predicted molecular weight: 57 kDa. Dilution optimised using Chromogenic detection.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).
  • Cellular localization
    Secreted protein; extracellular space.
  • Database links
  • Alternative names
    • 1 4 alpha D glucan glucanohydrolase antibody
    • 1,4 alpha D glucan glucanohydrolase 2B antibody
    • Alpha amylase 2B antibody
    • Alpha amylase antibody
    • AMY2 antibody
    • AMY2A antibody
    • AMY2B antibody
    • AMY3 antibody
    • Amylase alpha 2A antibody
    • Amylase alpha 2A pancreatic antibody
    • Amylase alpha 2B antibody
    • Amylase alpha 2B pancreatic antibody
    • Amylase pancreatic alpha 2A antibody
    • Amylase pancreatic alpha 2B antibody
    • Amylase, alpha 2A (pancreatic) antibody
    • Amylase, alpha 2B (pancreatic) antibody
    • Carcinoid alpha amylase antibody
    • Found in the pancreas antibody
    • Glycogenase antibody
    • HXA antibody
    • PA antibody
    • Pancreatic alpha amylase antibody
    • Pancreatic amylase 2A antibody
    • Pancreatic amylase alpha 2A antibody
    see all

Images

  • IHC image of Pancreatic Amylase staining in Human pancreas normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21156, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab21156 staining Pancreatic alpha amylase in mouse pancreas tissue in immunohistochemical analysis (Frozen sections).

    Tissue was blocked with 5% BSA for 30 minutes at 21°C. Samples were incubated with primary antibody for 1 hour at 21°C at 1/800 dilution. Renaissance background reducing diluent was used. An Alexa Fluor488-conjugated goat anti-rabbit IgG monoclonal (H+L) was used as secondary antibody at 1/200 dilution.

References

This product has been referenced in:
  • Swidnicka-Siergiejko AK  et al. Chronic inflammation initiates multiple forms of K-Ras-independent mouse pancreatic cancer in the absence of TP53. Oncogene 36:3149-3158 (2017). Read more (PubMed: 27991926) »
  • Richter M  et al. Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb) 5:278-290 (2016). Read more (PubMed: 30090344) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Purified protein (Pancreas)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
7.6 µg
Specification
Pancreas
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 22 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
no

Mrs. Sandy Piampiano

Verified customer

Submitted Feb 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Serum (serum)
Loading amount
30 µg
Specification
serum
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 24 2012

Answer

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and below)to mouse to expect any cross-reactivity. Unfortunately, this is also true for the other antibodies we offer against these targets too.

ab86704 is already tested and guaranteed for mouse and WB. Please reassure your customer that the Abpromise guarantee will apply to this antibody in their experiment.

DISCOUNT CODEs:

ab68715: xxxxx


ab86177: xxxxx


ab21156: xxxxx

Expiration date:17. May 2012

I am very pleased to hear your customer would like to accept our offer and test ab68715 and ab86177 and ab21156 in mouse.

The codes will give your customer 1 freeprimary antibody eachbefore the expiration date.

To redeem this offer, please submit an Abreview eachformouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. Generally we predict reactivity of an antibody if the immunogen sequence has 85% or greater homology with the species being tested. The immunogen for ab35414 and ab82890 is full length pig protein and for ab21156 it is full length human protein, but BLAST search results showed both of these beneath the 85% reactivity mark. We haven't tested ab35414 or ab21156 in ICC and have no data on whether they work in this application. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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