Overview

  • Product name

    Anti-Pancreatic alpha amylase antibody
    See all Pancreatic alpha amylase primary antibodies
  • Description

    Sheep polyclonal to Pancreatic alpha amylase
  • Host species

    Sheep
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Full length protein corresponding to Pig Pancreatic alpha amylase.

Properties

Applications

Our Abpromise guarantee covers the use of ab35414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/250 - 1/1000. Using 1 µg of antigen per well, the 50% OD was 1/500, and end point titre 1/50,000.
    WB: 1/5000. Detects a band of approximately 56 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).
    • Cellular localization

      Secreted protein; extracellular space.
    • Database links

    • Alternative names

      • 1 4 alpha D glucan glucanohydrolase antibody
      • 1,4 alpha D glucan glucanohydrolase 2B antibody
      • Alpha amylase 2B antibody
      • Alpha amylase antibody
      • alpha-amylase found in the pancreas antibody
      • AMY2 antibody
      • AMY2A antibody
      • AMY2B antibody
      • AMY3 antibody
      • Amylase alpha 2A antibody
      • Amylase alpha 2A pancreatic antibody
      • Amylase alpha 2B antibody
      • Amylase alpha 2B pancreatic antibody
      • Amylase pancreatic alpha 2A antibody
      • Amylase pancreatic alpha 2B antibody
      • Amylase, alpha 2A (pancreatic) antibody
      • Amylase, alpha 2B (pancreatic) antibody
      • Carcinoid alpha amylase antibody
      • carcinoid alpha-amylase antibody
      • Found in the pancreas antibody
      • Glycogenase antibody
      • HXA antibody
      • PA antibody
      • Pancreatic alpha amylase antibody
      • pancreatic alpha-amylase antibody
      • Pancreatic amylase 2A antibody
      • Pancreatic amylase alpha 2A antibody
      see all

    References

    ab35414 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. Generally we predict reactivity of an antibody if the immunogen sequence has 85% or greater homology with the species being tested. The immunogen for ab35414 and ab82890 is full length pig protein and for ab21156 it is full length human protein, but BLAST search results showed both of these beneath the 85% reactivity mark. We haven't tested ab35414 or ab21156 in ICC and have no data on whether they work in this application. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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