Recombinant Anti-Pancreatic Lipase/PTL antibody [EPR6275] (ab133556)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6275] to Pancreatic Lipase/PTL
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Pancreatic Lipase/PTL antibody [EPR6275]
See all Pancreatic Lipase/PTL primary antibodies -
Description
Rabbit monoclonal [EPR6275] to Pancreatic Lipase/PTL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human pancreas lysate, Human pancreas tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6275 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5406 Human
- Omim: 246600 Human
- SwissProt: P16233 Human
- Unigene: 501135 Human
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Alternative names
- lipase, pancreatic antibody
- LIPP_HUMAN antibody
- Pancreatic lipase antibody
see all
Images
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Anti-Pancreatic Lipase/PTL antibody [EPR6275] (ab133556) at 1/10000 dilution + Human pancreas lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
ab133556 showing negative staining in Normal brain tissue. -
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling Pancreatic Lipase/PTL with ab133556 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab133556 showing positive staining in Normal pancreas tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133556 has not yet been referenced specifically in any publications.