Recombinant Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6276] to Pancreatic Lipase/PTL
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Pancreatic Lipase/PTL antibody [EPR6276]
See all Pancreatic Lipase/PTL primary antibodies -
Description
Rabbit monoclonal [EPR6276] to Pancreatic Lipase/PTL -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Pancreatic Lipase/PTL aa 350-450. The exact sequence is proprietary.
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Positive control
- Human pancreas and Human small intestine lysates; Human pancreas tissue.
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General notes
Previously labelled as Pancreatic Lipase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.40 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6276 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5406 Human
- Omim: 246600 Human
- SwissProt: P16233 Human
- Unigene: 501135 Human
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Alternative names
- lipase, pancreatic antibody
- LIPP_HUMAN antibody
- Pancreatic lipase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
ab124915, at 1/500 dilution, staining Pancreatic Lipase/PTL in paraffin embedded Human pancreas tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915) at 1/10000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa
Protocols
References (0)
ab124915 has not yet been referenced specifically in any publications.