Recombinant
RabMAb

Recombinant Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)

Submit a review Q&A (2)

Overview

  • Product name

    Anti-Pancreatic Lipase/PTL antibody [EPR6276]
    See all Pancreatic Lipase/PTL primary antibodies

  • Description

    Rabbit monoclonal [EPR6276] to Pancreatic Lipase/PTL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Pancreatic Lipase/PTL aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Human pancreas and Human small intestine lysates; Human pancreas tissue.

  • General notes

     

     

    Previously labelled as Pancreatic Lipase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab124915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Sequence similarities

      Belongs to the AB hydrolase superfamily. Lipase family.
      Contains 1 PLAT domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • lipase, pancreatic antibody
        • LIPP_HUMAN antibody
        • Pancreatic lipase antibody
        • Pancreatic triacylglycerol lipase antibody
        • PL antibody
        • PNLIP antibody
        • PNLIPD antibody
        • PTL antibody
        • Triacylglycerol acylhydrolase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)

        ab124915, at 1/500 dilution, staining Pancreatic Lipase/PTL in paraffin embedded Human pancreas tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
        Western blot - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
        All lanes : Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915) at 1/10000 dilution

        Lane 1 : Human pancreas lysate
        Lane 2 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa

      • Other - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
        Other - Anti-Pancreatic Lipase/PTL antibody [EPR6276] (ab124915)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab124915 has not yet been referenced specifically in any publications.

      Publishing research using ab124915? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear Tech support

      Could you speculate, based on immunogen sequence, the chances of good antibody specificity against Rat for WB and IHC-P?

      Thank you very much for the support.
      Regards,

      Read More
      Answer

      Thank you for your enquiry.

      I am sorry to confirm that both Pancreatic Lipase antibodies you mention (ab124915, ab133556) are stated as 'does not react with mouse' and we would therefore not expect them to detect in this species,

      Regrettably as far as we are aware, non of the other pancreatic lipase antibodies has ever been tested in IHC-P or rat. All tested applications and tested species covered by the 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

      I can suggest to consider trying ab306746. The alignment of the immunogen with the rat sequence is high, and so we would predict this antibody would detect in rat. However, please note we are not able to guarantee this without further testing.

      If the customer would like to test ab306746 antibody in both rat and IHC-P, please do not hesitate to contact us again prior to the purchase by replying to this message as they will be eligible for our testing discount program.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

      Read More

      Question

      I tried to look at the datasheet of antibody ab306746 and got this answer:

      ab306746 doesn't match any products in our catalog. Try broadening your search or use alternative keywords.

      Did you mean to recommend another antibody?

      Thank you for the help.

      Regards,

      Read More
      Answer

      Thank you for your message.

      My apologies. This should have been ab30746:

      https://www.abcam.com/index.html?datasheet=30746 (or use the following: https://www.abcam.com/index.html?datasheet=30746).

      I hope this will be more helpful. Please do let me know if you have any further questions.

      Read More

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