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I suppose our customer is experiencing difficulty understanding the pdf file/attachment above in constructing an Eliza sandwich from using the two antibodies, ab37285 and ab37273.
The customer would like to know how to construct an Eliza sandwich using the two antibody pairing above, would you be able to recommend the method, should there be any other way different to the attached file above. (or do you think the file attachment above is not an appropriate instruction to follow)?
Asked on Jun 15 2012
Thank you for your email.
I also have heard back from the supplying lab who let me know thatab37285 was used as capture antibody at 1 µg/ml, and thatbiotinylated ab37273 (which is however notoffered by supplying lab) was used as detection antibody at 0.1 µg/ml. These are only guidelines for the dilutions. I do not have any further protocol information.
We have several conjugation kits that the customer could choose from (https://www.abcam.com/EasyLink). The biotinylation would not be exclusive, and other conjugations can be used as well - depending on the detection system the customer has available to him.
For biotin we have for instance EasyLink Biotin (type A) Conjugation Kit (3 x 100µg Biotin) (ab102864): https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html
We do not list sandwich ELISA as tested application as the antibodies per se (unconjugated) were not tested in this way. I can offer your participation in our testing discount program if the customer would like to try the antibodies in sandwich ELISA.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.
The protocol should be more clear now that the customer knows which antibody is for capturing and for detection. The secondary antibody will need to be left out as the detection antibody should conjugated.
I hope this answers all the questions. Please let me know if I can be offurther help.
Answered on Jun 15 2012