Recombinant Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22853-61] to Pancreatic Polypeptide - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr, mIHC
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free
See all Pancreatic Polypeptide primary antibodies -
Description
Rabbit monoclonal [EPR22853-61] to Pancreatic Polypeptide - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, mIHCmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse pancreas tissue; rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; rat pancreas tissue. mIHC: Rat pancreas tissue.
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General notes
ab256592 is the carrier-free version of ab255827.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22853-61 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
mIHC |
1/20000.
|
Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
mIHC
1/20000. |
Target
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Relevance
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 19064 Mouse
- Entrez Gene: 24677 Rat
- SwissProt: P10601 Mouse
- SwissProt: P06303 Rat
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Alternative names
- Pancreatic polypeptide antibody
- Pancreatic polypeptide Y antibody
- Pancreatic prohormone antibody
see all
Images
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This data was developed using the same antibody clone in a different buffer formulation (ab255827).
Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue.
Panel A: Merged staining of anti-Pancreatic Polypeptide (ab255827, green), anti-Somatostatin 28 (ab111912, red) and DAPI (blue).
Panel B: Anti-Pancreatic Polypeptide (green) stained on PP cells in rat pancreas islet.
Panel C: Anti-Somatostatin 28 (red) stained on delta cells in rat pancreas islet.
Panel D: Nuclear counter stain, DAPI (blue).
Key protocol steps: The section was incubated in two rounds of staining with ab255827 (1/20000 dilution) and ab111912 (1/2000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.
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Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells in rat pancreas islet (PMID: 27270601) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255827 for 10 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).
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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells in mouse pancreas islet (PMID: 27270601) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255827 for 10 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).
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Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). ab255827 and ab6995 are shown to stain PP cells and beta cells of rat pancreatic islets respectively (PMID: 24825414). The nuclear counter stain is DAPI (blue). Insulin is detected using ab6995, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).
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Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). ab255827 and ab6995 are shown to stain PP cells and beta cells of mouse pancreatic islet respectively (PMID: 24825414). The nuclear counter stain is DAPI (blue). Insulin is detected using ab6995, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab256592 has not yet been referenced specifically in any publications.