Anti-PANDER antibody (ab224100)
Key features and details
- Rabbit polyclonal to PANDER
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PANDER antibody
See all PANDER primary antibodies -
Description
Rabbit polyclonal to PANDER -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PANDER aa 50-200.
Database link: P58499 -
Positive control
- WB: PANDER overexpressing HEK-293T cell lysate. IHC-P: Human duodenum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin. -
Tissue specificity
Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis. -
Sequence similarities
Belongs to the FAM3 family. -
Post-translational
modifications2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. -
Cellular localization
Secreted. Localized in discrete vesicular and perinuclear structure. - Information by UniProt
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Database links
- Entrez Gene: 54097 Human
- Omim: 608617 Human
- SwissProt: P58499 Human
- Unigene: 473877 Human
- Unigene: 670704 Human
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Alternative names
- 2-21 antibody
- C21orf11 antibody
- C21orf76 antibody
see all
Images
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Paraffin embedded human duodenum tissue stained for PANDER with ab224100 (1/50) in immunohistochemical analysis.
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All lanes : Anti-PANDER antibody (ab224100) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : PANDER overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224100 has not yet been referenced specifically in any publications.