Product nameAnti-Pannexin 1 antibody
See all Pannexin 1 primary antibodies
DescriptionRabbit polyclonal to Pannexin 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Pig, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human Pannexin 1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: Human brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).|
FunctionStructural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
Sequence similaritiesBelongs to the pannexin family.
Cellular localizationCell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
- Information by UniProt
- innexin antibody
- MRS1 antibody
- Pannexin 1 antibody
Anti-Pannexin 1 antibody (ab124131) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of Pannexin 1 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 45 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This product has been referenced in:
- Li S et al. Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Exp Ther Med 15:2798-2803 (2018). Read more (PubMed: 29456683) »
- Zhou L et al. Pannexin-1 is involved in neuronal apoptosis and degeneration in experimental intracerebral hemorrhage in rats. Mol Med Rep 17:5684-5691 (2018). Read more (PubMed: 29484398) »