Key features and details
- Rabbit polyclonal to Pannexin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pannexin 1 antibody
See all Pannexin 1 primary antibodies
DescriptionRabbit polyclonal to Pannexin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human Pannexin 1 aa 228-411. (Expressed in E.coli).
CIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLS VINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYND LSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDV VDGKTPMSAEMREEQGNQTAELQGMNIDSETKAN
Database link: Q96RD7
- IHC-P: Human liver, liver cancer and kidney tissues. WB: SK-N-SH and U-87 MG cell lysates; Recombinant human Pannexin 1 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233479 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.|
FunctionStructural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
Sequence similaritiesBelongs to the pannexin family.
Cellular localizationCell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
- Information by UniProt
- innexin antibody
- MRS1 antibody
- Pannexin 1 antibody
Paraffin-embedded human liver tissue stained for Pannexin 1 using ab233479 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver cancer tissue stained for Pannexin 1 using ab233479 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Pannexin 1 using ab233479 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Pannexin 1 antibody (ab233479) at 3 µg/ml + Recombinant human Pannexin 1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 48 kDa
All lanes : Anti-Pannexin 1 antibody (ab233479) at 3 µg/ml
Lane 1 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 48 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233479 has not yet been referenced specifically in any publications.