Anti-Pannexin 1 antibody [EPR5556] (ab124969)


  • Product name
    Anti-Pannexin 1 antibody [EPR5556]
    See all Pannexin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5556] to Pannexin 1
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal brain tissue lysate and SH-SY5Y, Caco2, and NIH OVCAR-3 cell lysates. IP: SH-SY5Y cell lysate.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)
    KD = 1.52 x 10 -10 M
    Learn more about KD
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab124969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
    • Sequence similarities
      Belongs to the pannexin family.
    • Cellular localization
      Cell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • innexin antibody
      • MRS1 antibody
      • Pannexin 1 antibody
      • Pannexin-1 antibody
      • Panx1 antibody
      • PANX1_HUMAN antibody
      • PX1 antibody
      • UNQ2529 antibody
      see all


    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)

      Lane 1 : Caco-2 whole cell lysate
      Lane 2 : NIH:OVCAR-3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate (10µg)

      Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal brain cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco2 cell lysates
      Lane 4 : NIH OVCAR-3 cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 48 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Skals M  et al. Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore. J Biol Chem 289:19098-109 (2014). WB ; Mouse . Read more (PubMed: 24860098) »

    See 1 Publication for this product

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