Product nameAnti-Pannexin 1 antibody [EPR5556]
See all Pannexin 1 primary antibodies
DescriptionRabbit monoclonal [EPR5556] to Pannexin 1
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.
- WB: Human fetal brain tissue lysate and SH-SY5Y, Caco2, and NIH OVCAR-3 cell lysates. IP: SH-SY5Y cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.52 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab124969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
FunctionStructural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
Sequence similaritiesBelongs to the pannexin family.
Cellular localizationCell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
- Information by UniProt
- innexin antibody
- MRS1 antibody
- Pannexin 1 antibody
All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)
Lane 1 : Caco-2 whole cell lysate
Lane 2 : NIH:OVCAR-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Blocking and dilution buffer: 5% NFDM /TBST.
ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y whole cell lysate (10µg)
Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : Caco2 cell lysates
Lane 4 : NIH OVCAR-3 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 48 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Chen W et al. Enhanced Macrophage Pannexin 1 Expression and Hemichannel Activation Exacerbates Lethal Experimental Sepsis. Sci Rep 9:160 (2019). Read more (PubMed: 30655582) »
- Skals M et al. Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore. J Biol Chem 289:19098-109 (2014). WB ; Mouse . Read more (PubMed: 24860098) »