Overview

  • Product name

    Anti-Pantothenate kinase 4 antibody
    See all Pantothenate kinase 4 primary antibodies
  • Description

    Rabbit polyclonal to Pantothenate kinase 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Pantothenate kinase 4 aa 50-100.
    Database link: NP_060686.1

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates
  • General notes

    Previously labelled as PANK4. 

Properties

Applications

Our Abpromise guarantee covers the use of ab137243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    Pantothenate kinase 4 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the physiological regulation of the intracellular coenzyme A (CoA) concentration. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • DKFZp547M242 antibody
    • FLJ10782 antibody
    • hPanK 4 antibody
    • hPanK4 antibody
    • PANK 4 antibody
    • Pantothenate kinase 4 antibody
    • Pantothenic acid kinase antibody
    see all

Images

  • All lanes : Anti-Pantothenate kinase 4 antibody (ab137243) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 86 kDa


    Exposure time: 30 seconds
  • Detection of Pantothenate kinase 4 by Western blot of immunoprecipitate
    ab137243, at 6 µg/ml, immunoprecipitating Pantothenate kinase 4 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    1 µg/ml of ab137243 was used for the subsequent Western blot.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab137243 has not yet been referenced specifically in any publications.

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