Product nameAnti-Pantothenate kinase 4 antibody
See all Pantothenate kinase 4 primary antibodies
DescriptionRabbit polyclonal to Pantothenate kinase 4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to Human Pantothenate kinase 4 aa 50-100.
Database link: NP_060686.1
- 293T, HeLa and Jurkat whole cell lysates
Previously labelled as PANK4.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab137243 was affinity purified using an epitope specific to Pantothenate kinase 4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab137243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevancePantothenate kinase 4 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the physiological regulation of the intracellular coenzyme A (CoA) concentration. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues.
- DKFZp547M242 antibody
- FLJ10782 antibody
- hPanK 4 antibody
All lanes : Anti-Pantothenate kinase 4 antibody (ab137243) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds
Detection of Pantothenate kinase 4 by Western blot of immunoprecipitate
ab137243, at 6 µg/ml, immunoprecipitating Pantothenate kinase 4 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
1 µg/ml of ab137243 was used for the subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab137243 has not yet been referenced specifically in any publications.