Anti-Pantothenate kinase 4 (phospho S63) antibody (ab228514)
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Overview
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Product name
Anti-Pantothenate kinase 4 (phospho S63) antibody
See all Pantothenate kinase 4 primary antibodies -
Description
Rabbit polyclonal to Pantothenate kinase 4 (phospho S63) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Hamster
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Immunogen
Synthetic peptide corresponding to Mouse Pantothenate kinase 4 (phospho S63).
Database link: Q80YV4 -
Positive control
- WB: Mouse whole brain lysate.
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General notes
Previously labelled as PANK4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab228514 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 92 kDa. |
Target
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Relevance
Pantothenate kinase 4 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the physiological regulation of the intracellular coenzyme A (CoA) concentration. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 269614 Mouse
- Entrez Gene: 171053 Rat
- SwissProt: Q80YV4 Mouse
- SwissProt: Q923S8 Rat
- Unigene: 30042 Mouse
- Unigene: 17321 Rat
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Alternative names
- DKFZp547M242 antibody
- FLJ10782 antibody
- hPanK 4 antibody
see all
Images
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Western blot - Anti-Pantothenate kinase 4 (phospho S63) antibody (ab228514)All lanes : Anti-Pantothenate kinase 4 (phospho S63) antibody (ab228514) at 1/1000 dilution
Lane 1 : Mouse whole brain lysate
Lane 2 : Mouse whole brain lysate treated with lambda phosphatase (1200 units for 30 minutes).
Predicted band size: 92 kDaPhosphospecificity is shown in Lane 2 where immunolabeling is nearly eliminated with lambda phosphatase (1200 units for 30 minutes).
Datasheets and documents
References
ab228514 has not yet been referenced specifically in any publications.
