Key features and details
- Rabbit polyclonal to Pantothenate kinase 4 (phospho S63)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Pantothenate kinase 4 (phospho S63) antibody
See all Pantothenate kinase 4 primary antibodies
DescriptionRabbit polyclonal to Pantothenate kinase 4 (phospho S63)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Hamster
Synthetic peptide corresponding to Mouse Pantothenate kinase 4 (phospho S63).
Database link: Q80YV4
- WB: Mouse whole brain lysate.
Previously labelled as PANK4.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.24% HEPES, 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228514 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Our Abpromise guarantee covers the use of ab228514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 92 kDa.|
RelevancePantothenate kinase 4 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the physiological regulation of the intracellular coenzyme A (CoA) concentration. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues.
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All lanes : Anti-Pantothenate kinase 4 (phospho S63) antibody (ab228514) at 1/1000 dilution
Lane 1 : Mouse whole brain lysate
Lane 2 : Mouse whole brain lysate treated with lambda phosphatase (1200 units for 30 minutes).
Predicted band size: 92 kDa
Phosphospecificity is shown in Lane 2 where immunolabeling is nearly eliminated with lambda phosphatase (1200 units for 30 minutes).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228514 has not yet been referenced specifically in any publications.