Key features and details
- Rabbit polyclonal to PAOX/PAO
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-PAOX/PAO antibody
See all PAOX/PAO primary antibodies
DescriptionRabbit polyclonal to PAOX/PAO
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human PAOX/PAO aa 130-356. Two N-terminal tags. (Expressed in E.coli).
IERTREFLNESETPMASVGEFLKKEISQQVASWTEDDEDTRKRKLAILNT FFNIECCVSGTHSMDLVALAPFGEYTVLPGLDCILAGGYQGLTDRILASL PKDTVAFDKPVKTIHWNGSFQEAAFPGETFPVLVECEDGARLPAHHVIVT VPLGFLKEHQDTFFEPPLPAKKAEAIKKLGFGTNNKIFLEFEEPFWEPDC QFIQVVWEDTSPLQDTALSLQDTWFKK
Database link: Q8C0L6
- WB: Mouse pancreas lysate; Recombinant mouse PAOX/PAO protein. IHC-P: Mouse testis tissue.
This product was previously labelled as PAOX
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevancePAOX is a flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. It can also oxidize N(1)-acetylspermidine to putrescine. It does not oxidize spermidine. PAOX plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. There are eleven named isoforms.
Cellular localizationPeroxisome. Cytoplasm.
- DKFZp434J245 antibody
- MGC45464 antibody
- PAO antibody
Formalin-fixed, paraffin-embedded mouse testis tissue stained for PAOX/PAO using ab233476 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-PAOX/PAO antibody (ab233476) at 3 µg/ml + Mouse pancreas tissue lysate
HRP-Linked Guinea pig anti-rabbit at 2000 mg/ml
Anti-PAOX/PAO antibody (ab233476) at 3 µg/ml + Recombinant mouse PAOX/PAO protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233476 has not yet been referenced specifically in any publications.