Overview

  • Product name

  • Description

    Goat polyclonal to PAP
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human PAP aa 247-259 (internal sequence).
    Sequence:

    C-YGIHKQKEKSRLQ

  • Positive control

    • Human Prostate Lysate
  • General notes

     This product was previously labelled as Prostatic Acid Phosphatase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab103893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-PAP antibody (ab103893) at 0.3 µg/ml + Human Prostate Lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • ab103893 (3.8µg/ml) staining of paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab103893 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab103893.
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