Overview

  • Product name

    Anti-PAP antibody [EPR4066]
    See all PAP primary antibodies
  • Description

    Rabbit monoclonal [EPR4066] to PAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human PAP aa 1-100.

  • Positive control

    • Prostate cancer cell lysate, human prostatic and human prostatic adenocarcinoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Prostatic Acid Phosphatase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities

      Belongs to the histidine acid phosphatase family.
    • Cellular localization

      Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' NT antibody
      • 5' nucleotidase antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • Acid phosphatase prostate antibody
      • ACP 3 antibody
      • ACP3 antibody
      • acpP antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • PAP antibody
      • PPAP_HUMAN antibody
      • Prostatic acid phosphatase antibody
      • Prostatic acid phosphotase antibody
      • Thiamine monophosphatase antibody
      • TMPase antibody
      see all

    Images

    • Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-PAP (ab108984).

    • Anti-PAP antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

      Predicted band size: 45 kDa

    • Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-PAP (ab108984).

    • ab108984 showing negative staining in Skeletal muscle tissue.

    • ab108984 showing negative staining in Colonic adenocarcinoma tissue.

    • ab108984 showing negative staining in Normal tonsil tissue.

    • ab108984 showing negative staining in Normal placenta tissue.

    • ab108984 showing positive staining in Prostatic carcinoma tissue.

    • ab108984 showing negative staining in Breast carcinoma tissue.

    • ab108984 showing negative staining in Cervical carcinoma tissue.

    • ab108984 showing negative staining in Normal breast tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Liu T  et al. FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness. Proc Natl Acad Sci U S A 114:7683-7688 (2017). Read more (PubMed: 28674022) »
    • Wei J  et al. Rapid progression of mixed neuroendocrine carcinoma-acinar adenocarcinoma of the prostate: A case report. Oncol Lett 12:1019-1022 (2016). IHC-P ; Human . Read more (PubMed: 27446387) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate Cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA based buffer pH 9.0
    Permeabilization
    No
    Specification
    Prostate Cancer
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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