Recombinant Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
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Overview
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Product name
Anti-PAP antibody [EPR4066] - BSA and Azide free
See all PAP primary antibodies -
Description
Rabbit monoclonal [EPR4066] to PAP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PAP aa 1-100. The exact sequence is proprietary.
Database link: P15309 -
General notes
ab247732 is the carrier-free version of ab108984 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247732 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Prostatic Acid Phosphatase
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.41 x 10 -10 M Learn more about KD -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4066 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. | |
| IP | Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the histidine acid phosphatase family. -
Cellular localization
Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles. - Information by UniProt
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Database links
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
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Alternative names
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
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Datasheets and documents
References
ab247732 has not yet been referenced specifically in any publications.
