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RecombinantRabMAb

Recombinant Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Western blot - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • OI-RD Scanning - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
  • Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4066] to PAP - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-PAP antibody [EPR4066] - BSA and Azide free
    See all PAP primary antibodies
  • Description

    Rabbit monoclonal [EPR4066] to PAP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PAP aa 1-100. The exact sequence is proprietary.
    Database link: P15309

  • General notes

    ab247732 is the carrier-free version of ab108984 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247732 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Prostatic Acid Phosphatase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.41 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4066
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • Kallikreins
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Phosphatases

Associated products

  • Alternative Versions

    • Anti-PAP antibody [EPR4066] (ab108984)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant human PAP protein (Active) (ab219224)

Applications

Our Abpromise guarantee covers the use of ab247732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities

      Belongs to the histidine acid phosphatase family.
    • Cellular localization

      Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Target information above from: UniProt accession P15309 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55 Human
      • Omim: 171790 Human
      • SwissProt: P15309 Human
      • Unigene: 433060 Human
      • Alternative names

        • 5' NT antibody
        • 5' nucleotidase antibody
        • 5'-NT antibody
        • 5'-nucleotidase antibody
        • Acid phosphatase prostate antibody
        • ACP 3 antibody
        • ACP3 antibody
        • acpP antibody
        • Ecto 5' nucleotidase antibody
        • Ecto-5'-nucleotidase antibody
        • PAP antibody
        • PPAP_HUMAN antibody
        • Prostatic acid phosphatase antibody
        • Prostatic acid phosphotase antibody
        • Thiamine monophosphatase antibody
        • TMPase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-PAP (ab108984). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Western blot - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Western blot - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Anti-PAP antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

        Predicted band size: 45 kDa



        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-PAP (ab108984). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        ab108984 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        ab108984 showing negative staining in Colonic adenocarcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        ab108984 showing negative staining in Normal tonsil tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

        This data was developed using ab108984, the same antibody clone in a different buffer formulation.

        ab108984 showing negative staining in Normal placenta tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        This data was developed using ab108984, the same antibody clone in a different buffer formulation.ab108984 showing positive staining in Prostatic carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        This data was developed using ab108984, the same antibody clone in a different buffer formulation.ab108984 showing negative staining in Breast carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        This data was developed using ab108984, the same antibody clone in a different buffer formulation.ab108984 showing negative staining in Cervical carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        This data was developed using ab108984, the same antibody clone in a different buffer formulation.ab108984 showing negative staining in Normal breast tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • OI-RD Scanning - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        OI-RD Scanning - Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        This data was developed using ab108984, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)
        Anti-PAP antibody [EPR4066] - BSA and Azide free (ab247732)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247732? Please let us know so that we can cite the reference in this datasheet.

      ab247732 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab247732.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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