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    pap-antibody-lt3d1-ab61707.pdf

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Anti-PAP antibody [LT3D1] (ab61707)

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Flow Cytometry - Anti-PAP antibody [LT3D1] (ab61707)

    Key features and details

    • Mouse monoclonal [LT3D1] to PAP
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
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    Recombinant human PAP protein (Active) (ab219224)

    View more associated products

    Overview

    • Product name

      Anti-PAP antibody [LT3D1]
      See all PAP primary antibodies
    • Description

      Mouse monoclonal [LT3D1] to PAP
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Other Immunogen Type corresponding to PAP. Genetic immunisation with cDNA encoding human PAP (PAcP, ACPP). Selection based on recognition of the complete native protein expressed on transfected mammalian cells.

    • General notes

       This product was previously labelled as Prostatic Acid Phosphatase

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      LT3D1
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Serine protease
      • Kallikreins
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • Phosphatases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human PAP protein (Active) (ab219224)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab61707 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Notes
  • Application notes
    cELISA: 1/200 - 1/400.
    Flow Cyt: Use 1.2µg for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities

      Belongs to the histidine acid phosphatase family.
    • Cellular localization

      Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Target information above from: UniProt accession P15309 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55 Human
      • Omim: 171790 Human
      • SwissProt: P15309 Human
      • Unigene: 433060 Human
      • Alternative names

        • 5' NT antibody
        • 5' nucleotidase antibody
        • 5'-NT antibody
        • 5'-nucleotidase antibody
        • Acid phosphatase prostate antibody
        • ACP 3 antibody
        • ACP3 antibody
        • acpP antibody
        • Ecto 5' nucleotidase antibody
        • Ecto-5'-nucleotidase antibody
        • PAP antibody
        • PPAP_HUMAN antibody
        • Prostatic acid phosphatase antibody
        • Prostatic acid phosphotase antibody
        • Thiamine monophosphatase antibody
        • TMPase antibody
        see all

      Images

      • Flow Cytometry - Anti-PAP antibody [LT3D1] (ab61707)
        Flow Cytometry - Anti-PAP antibody [LT3D1] (ab61707)
        Ab 61707 staining of BOSC23 cells transiently transfected with an expression vector encoding either PAP (red curve) or an irrelevantprotein (control transfectant: black curve). Binding of ab61707 was detected with a PE-conjugated secondary antibody. A positivesignal was obtained only with PAP transfected cells

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab61707? Please let us know so that we can cite the reference in this datasheet.

      ab61707 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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