Anti-PAP antibody [LT3D1] (ab61707)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PAP antibody [LT3D1]
See all PAP primary antibodies -
Description
Mouse monoclonal [LT3D1] to PAP -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, Competitive ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to PAP. Genetic immunisation with cDNA encoding human PAP (PAcP, ACPP). Selection based on recognition of the complete native protein expressed on transfected mammalian cells.
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General notes
This product was previously labelled as Prostatic Acid Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
LT3D1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab61707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | ||
| Competitive ELISA |
Application notes
cELISA: 1/200 - 1/400.
Flow Cyt: Use 1.2µg for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1.2µg for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Sequence similarities
Belongs to the histidine acid phosphatase family. -
Cellular localization
Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles. - Information by UniProt
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Database links
- Entrez Gene: 55 Human
- Omim: 171790 Human
- SwissProt: P15309 Human
- Unigene: 433060 Human
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Alternative names
- 5' NT antibody
- 5' nucleotidase antibody
- 5'-NT antibody
see all
Images
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![Flow Cytometry - Anti-PAP antibody [LT3D1] (ab61707)](https://www.abcam.com/ps/products/61/ab61707/Images/61707_1 copy.jpg)
Flow Cytometry - Anti-PAP antibody [LT3D1] (ab61707)Ab 61707 staining of BOSC23 cells transiently transfected with an expression vector encoding either PAP (red curve) or an irrelevantprotein (control transfectant: black curve). Binding of ab61707 was detected with a PE-conjugated secondary antibody. A positivesignal was obtained only with PAP transfected cells
Protocols
Datasheets and documents
References
ab61707 has not yet been referenced specifically in any publications.
