Overview

  • Product name
  • Description
    Goat polyclonal to PAPD5
  • Host species
    Goat
  • Specificity
    ab118341 is expected to recognize both reported isoforms (NP_001035374.2; NP_001035375.2).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human PAPD5 aa 497-510 (internal sequence) (Cysteine residue). NP_001035374.2; NP_001035375.2
    Sequence:

    TDEVATYRDWISKQ

  • Positive control
    • WB: Mouse and rat spleen lysate.
  • General notes

    Protein previously known as TRF4-2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab118341 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • Relevance
    Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • PAP-associated domain-containing protein 5 antibody
    • PAPD5 antibody
    • Poly(A) RNA polymerase D5 non canonical antibody
    • Terminal uridylyltransferase 3 antibody
    • Topoisomerase related function protein 4-2 antibody
    • TRF4-2 antibody
    • TUTase 3 antibody
    see all

Images

  • Anti-PAPD5 antibody (ab118341) at 0.2 µg/ml (for 1 hour) + Mouse spleen lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 63 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

References

ab118341 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab118341.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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