Key features and details
- Rabbit polyclonal to PAPOLA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAPOLA antibody
See all PAPOLA primary antibodies
DescriptionRabbit polyclonal to PAPOLA
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residue 694 and the C-terminus (residue 744) of human PAPOLA (NP_116021.2)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
Our Abpromise guarantee covers the use of ab72492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
FunctionPolymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
Sequence similaritiesBelongs to the poly(A) polymerase family.
modificationsPolysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
Cellular localizationCytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
- Information by UniProt
- MGC5378 antibody
- PAP alpha antibody
- PAP antibody
ab72492 has been referenced in 1 publication.
- Sagawa F et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 30:3994-4005 (2011). PubMed: 21822216