Overview

  • Product name

  • Description

    Rabbit polyclonal to PAPOLA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 694 and the C-terminus (residue 744) of human PAPOLA (NP_116021.2)

  • Positive control

    • HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    • Sequence similarities

      Belongs to the poly(A) polymerase family.
    • Post-translational
      modifications

      Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
      Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
      Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
    • Cellular localization

      Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
    • Information by UniProt
    • Database links

    • Alternative names

      • MGC5378 antibody
      • PAP alpha antibody
      • PAP antibody
      • PAP-alpha antibody
      • PapIII antibody
      • PAPOA_HUMAN antibody
      • PAPOLA antibody
      • Plap antibody
      • Poly(A) polymerase alpha antibody
      • Polynucleotide adenylyltransferase alpha antibody
      see all

    Images

    • All lanes : Anti-PAPOLA antibody (ab72492) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Predicted band size: 83 kDa
      Observed band size: 83 kDa


      Exposure time: 3 minutes

    References

    This product has been referenced in:

    • Sagawa F  et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 30:3994-4005 (2011). Read more (PubMed: 21822216) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for the protocol and result details. Regarding a replacement, there have not been other complaints about the antibody but not many people have purchased it. We have only the lot that you received, and I am concerned that another vial will simply give you the same result. I think a better choice for a replacement is ab126934:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=126934).

    Please let me know if you are interested in trying this. Our guarantee will also apply to this antibody, if tested on human samples in western blots. If you would rather have a refund or credit, I can issue that instead.

    Read More

    Answer

    Thank you for your reply. I have found your order in our system.

    Could you please let me know also the following details:

    1) What antibody dilution and incubation time was used?
    2) What was used for blocking?
    3) You reported no signal: Did you see any other bands, or was the blot completely empty?
    4) I assume the secondary antibody was HRP-conjugated, and is working well otherwise?
    5) What was your positive control antibody?

    The options weoffer are: a free of charge replacement with another PAPOLA antibody or an antibody of your choice (e.g. to a different target protein), a credit or a refund.

    Please let me know which option you would prefer.

    I look forward to hear back from you and resolve this case.

    Read More

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