Anti-PAPOLA antibody - C-terminal (ab231272)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PAPOLA antibody - C-terminal
See all PAPOLA primary antibodies -
Description
Rabbit polyclonal to PAPOLA - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PAPOLA (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P51003 -
Positive control
- WB: Nuclear extracts from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab231272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 83 kDa. |
Target
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Function
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus. -
Sequence similarities
Belongs to the poly(A) polymerase family. -
Post-translational
modificationsPolysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex. -
Cellular localization
Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 10914 Human
- Omim: 605553 Human
- SwissProt: P51003 Human
- Unigene: 253726 Human
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Alternative names
- MGC5378 antibody
- PAP alpha antibody
- PAP antibody
see all
Images
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Western blot - Anti-PAPOLA antibody - C-terminal (ab231272)Anti-PAPOLA antibody - C-terminal (ab231272) at 1/1000 dilution + Nuclear extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells at 40 µg
Predicted band size: 83 kDaDiliution buffer: TBS-Tween containing 5% skimmed milk.
Datasheets and documents
References
ab231272 has not yet been referenced specifically in any publications.
