Overview

  • Product name

    Anti-PAPP A antibody [9]
    See all PAPP A primary antibodies
  • Description

    Mouse monoclonal [9] to PAPP A
  • Host species

    Mouse
  • Specificity

    This antibody is specific for native human PAPP A. Heat and SDS denatured PAPP A is also recognized, but not acid treated PAPP A.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human)

  • Epitope

    The epitope is located on the PAPP A subunit of the heterotetramer.
  • General notes

    Clone number also known as [004-09]

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purification notes

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    9
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 181 kDa.
ELISA 1/8000. This antibody can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP A in coat.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
  • Tissue specificity

    High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
  • Sequence similarities

    Belongs to the peptidase M43B family.
    Contains 5 Sushi (CCP/SCR) domains.
  • Developmental stage

    Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
  • Post-translational
    modifications

    There appear to be no free sulfhydryl groups.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASBABP2 antibody
    • Aspecific BCL2 ARE binding protein 2 antibody
    • Differentially placenta 1 expressed protein antibody
    • DIPLA1 antibody
    • EC 3.4.24.79 antibody
    • IGF dependent IGFBP 4 protease antibody
    • IGF-dependent IGFBP-4 protease antibody
    • IGFBP 4ase antibody
    • IGFBP-4ase antibody
    • IGFBP4ase antibody
    • Insulin like growth factor dependent IGF binding protein 4 protease antibody
    • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
    • PAPA antibody
    • PAPP A antibody
    • PAPP-A antibody
    • PAPP1_HUMAN antibody
    • PAPPA antibody
    • PAPPA1 antibody
    • Pappalysin 1 precursor antibody
    • Pappalysin-1 antibody
    • Pregnancy Associated Plasma Protein A antibody
    • Pregnancy associated plasma protein A pappalysin 1 antibody
    • Pregnancy-associated plasma protein A antibody
    see all

Images

  • Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using frozen tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.
  • Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using parrafin embedded tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.

References

This product has been referenced in:

  • Sun Y  et al. MiR-490-3p modulates the proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A. Cardiovasc Res 100:272-9 (2013). Read more (PubMed: 23821525) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you very much for your call today, and I am very sorry for the delay in getting back to you.

I'm sending a free of charge vial of ab52030 on the order ***, which should arrive tomorrow.

Please keep us updated about the results with this replacement antibody, and let me know if there is anything else that we can do for you.

Read More

Answer

Thank you for your inquiry.

ab59086: Five different pappalysin-1 sequences are published thus far; 1628, 1627, 1420, 1232 and 704 amino acids in length. The shorter forms start with the second methionine, 209 residues downstream from the long forms, after the LamGL domain. The 1232 sequence lacks the final Notch/Lin domain, the 720 sequence ends after the catalytic domain. The 1628/7, 1420, 1232 and 720 forms have predicted masses of 181, 157.9, 137.1 and 78.7 kDa as monomers, respectively. ab59086 recognizes the 1627/8 amino acid pappalysin-1, but not the shorter forms.

ab52030: I can confirm that the immunogen for ab52030full length immunogen. This antibody is amouse monoclonal and therefore binds only once per protein molecule. The epitope is located on the PAPP A subunit of the heterotetramer. The epitope has not been mapped further.

I hope this information is helpful and wish you good luck with your experiments.

Read More

Answer

Thank you for your reply.

Iam sorry to confirm thatwe do not have the requested information.

The immunogen used to raise ab52030 was PAPP-A purified from fluid containing maternal serum aspirated during cesarean section (“cesarean fluid”) using heparin affinity chromatography followed by ionexchange chromatography.

NMRI-BALBc mice were injected subcutaneous with the purified PAPP-A in incomplete Freund’s adjuvant.

The purified PAPP-A was not sequenced.

I hope this information will be nevertheless helpful and wish you good luck with your research.

Read More

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