Recombinant Anti-PAPP A antibody [EPR12897] (ab174314)


  • Product name

    Anti-PAPP A antibody [EPR12897]
    See all PAPP A primary antibodies
  • Description

    Rabbit monoclonal [EPR12897] to PAPP A
  • Host species

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAPP A aa 1600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13219

  • Positive control

    • Human placenta lysate. Human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab174314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
    • Tissue specificity

      High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
    • Sequence similarities

      Belongs to the peptidase M43B family.
      Contains 5 Sushi (CCP/SCR) domains.
    • Developmental stage

      Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
    • Post-translational

      There appear to be no free sulfhydryl groups.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • ASBABP2 antibody
      • Aspecific BCL2 ARE binding protein 2 antibody
      • Differentially placenta 1 expressed protein antibody
      • DIPLA1 antibody
      • EC antibody
      • IGF dependent IGFBP 4 protease antibody
      • IGF-dependent IGFBP-4 protease antibody
      • IGFBP 4ase antibody
      • IGFBP-4ase antibody
      • IGFBP4ase antibody
      • Insulin like growth factor dependent IGF binding protein 4 protease antibody
      • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
      • PAPA antibody
      • PAPP A antibody
      • PAPP-A antibody
      • PAPP1_HUMAN antibody
      • PAPPA antibody
      • PAPPA1 antibody
      • Pappalysin 1 precursor antibody
      • Pappalysin-1 antibody
      • Pregnancy Associated Plasma Protein A antibody
      • Pregnancy associated plasma protein A pappalysin 1 antibody
      • Pregnancy-associated plasma protein A antibody
      see all


    • Anti-PAPP A antibody [EPR12897] (ab174314) at 1/1000 dilution + Human placenta lysate at 10 µg

      Predicted band size: 180 kDa

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with ab174314 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot analysis on immunoprecipitation pellet from Human placenta lysate using ab174314 at a 1/10 dilution.


    ab174314 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174314.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up