Recombinant
RabMAb

Anti-PAPP A antibody [EPR12897] (ab174314)

Overview

  • Product name
    Anti-PAPP A antibody [EPR12897]
    See all PAPP A primary antibodies
  • Description
    Rabbit monoclonal [EPR12897] to PAPP A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAPP A aa 1600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13219

  • Positive control
    • Human placenta lysate. Human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12897
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab174314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
    • Tissue specificity
      High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
    • Sequence similarities
      Belongs to the peptidase M43B family.
      Contains 5 Sushi (CCP/SCR) domains.
    • Developmental stage
      Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
    • Post-translational
      modifications
      There appear to be no free sulfhydryl groups.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASBABP2 antibody
      • Aspecific BCL2 ARE binding protein 2 antibody
      • Differentially placenta 1 expressed protein antibody
      • DIPLA1 antibody
      • EC 3.4.24.79 antibody
      • IGF dependent IGFBP 4 protease antibody
      • IGF-dependent IGFBP-4 protease antibody
      • IGFBP 4ase antibody
      • IGFBP-4ase antibody
      • IGFBP4ase antibody
      • Insulin like growth factor dependent IGF binding protein 4 protease antibody
      • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
      • PAPA antibody
      • PAPP A antibody
      • PAPP-A antibody
      • PAPP1_HUMAN antibody
      • PAPPA antibody
      • PAPPA1 antibody
      • Pappalysin 1 precursor antibody
      • Pappalysin-1 antibody
      • Pregnancy Associated Plasma Protein A antibody
      • Pregnancy associated plasma protein A pappalysin 1 antibody
      • Pregnancy-associated plasma protein A antibody
      see all

    Images

    • Anti-PAPP A antibody [EPR12897] (ab174314) at 1/1000 dilution + Human placenta lysate at 10 µg

      Predicted band size: 180 kDa

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAPP A with ab174314 at a 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from Human placenta lysate using ab174314 at a 1/10 dilution.

    References

    ab174314 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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