Overview

  • Product name

  • Description

    Rabbit polyclonal to PAPSS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human PAPSS1 (internal sequence). The exact sequence is proprietary.
    Database link: O43252

  • Positive control

    • WB: MCF7 whole cell lysate. IHC-P: Human colon carcinoma and gastric cancer tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
  • Tissue specificity

    Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.
  • Pathway

    Sulfur metabolism; sulfate assimilation.
  • Sequence similarities

    In the N-terminal section; belongs to the APS kinase family.
    In the C-terminal section; belongs to the sulfate adenylyltransferase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1 antibody
    • 3' phosphoadenosine 5' phosphosulfate synthase 1 antibody
    • 3''-phosphoadenosine-5''-phosphosulfate synthase antibody
    • Adenosine-5''-phosphosulfate 3''-phosphotransferase antibody
    • Adenylyl-sulfate kinase antibody
    • Adenylylsulfate 3''-phosphotransferase antibody
    • APS kinase antibody
    • ATP-sulfurylase antibody
    • ATPSK 1 antibody
    • ATPSK1 antibody
    • ATPSK2 antibody
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1 antibody
    • PAPS synthase 1 antibody
    • PAPS synthetase 1 antibody
    • PAPS1_HUMAN antibody
    • PAPSS 1 antibody
    • PAPSS antibody
    • PAPSS1 antibody
    • SAT antibody
    • SK 1 antibody
    • SK1 antibody
    • SK2 antibody
    • Sulfate adenylate transferase antibody
    • Sulfurylase kinase 1 antibody
    see all

Images

  • Anti-PAPSS1 antibody (ab228622) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 71 kDa



    7.5% SDS PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for PAPSS1 using ab228622 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human gastric cancer tissue stained for PAPSS1 using ab228622 at 1/500 dilution in immunohistochemical analysis.

References

ab228622 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab228622.
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