Anti-PAPSS2 antibody (ab155585)
Key features and details
- Rabbit polyclonal to PAPSS2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAPSS2 antibody
See all PAPSS2 primary antibodies -
Description
Rabbit polyclonal to PAPSS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 334-612 of Human PAPSS2 (Uniprot ID: O95340).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; A431 cells; Human H3347 xenograft tissue.
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General notes
Keep as a concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| IHC-P | 1/100 - 1/1000. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
PAPSS2 is one of the two PAPS synthetases. Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2. -
Database links
- Entrez Gene: 536409 Cow
- Entrez Gene: 9060 Human
- Entrez Gene: 23972 Mouse
- Entrez Gene: 294103 Rat
- Entrez Gene: 398910 Xenopus laevis
- Omim: 603005 Human
- SwissProt: O95340 Human
- SwissProt: O88428 Mouse
see all -
Alternative names
- 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
- 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
- ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
see all
Images
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Western blot - Anti-PAPSS2 antibody (ab155585)Anti-PAPSS2 antibody (ab155585) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PAPSS2 antibody (ab155585)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PAPSS2 with ab155585 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (ab155585)Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PAPSS2 with ab155585 at 1/500 dilution.
Protocols
References (0)
ab155585 has not yet been referenced specifically in any publications.
