Anti-PAPSS2 antibody (ab155585)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PAPSS2 antibody
See all PAPSS2 primary antibodies -
Description
Rabbit polyclonal to PAPSS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 334-612 of Human PAPSS2 (Uniprot ID: O95340).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; A431 cells; Human H3347 xenograft tissue.
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General notes
Keep as a concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| IHC-P | 1/100 - 1/1000. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
PAPSS2 is one of the two PAPS synthetases. Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2. -
Database links
- Entrez Gene: 536409 Cow
- Entrez Gene: 9060 Human
- Entrez Gene: 23972 Mouse
- Entrez Gene: 294103 Rat
- Entrez Gene: 398910 Xenopus laevis
- Omim: 603005 Human
- SwissProt: O95340 Human
- SwissProt: O88428 Mouse
see all -
Alternative names
- 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
- 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
- ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
see all
Images
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Western blot - Anti-PAPSS2 antibody (ab155585)Anti-PAPSS2 antibody (ab155585) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PAPSS2 antibody (ab155585)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PAPSS2 with ab155585 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAPSS2 antibody (ab155585)Immunohistochemical analysis of paraffin-embedded Human H3347 xenograft tissue labeling PAPSS2 with ab155585 at 1/500 dilution.
Protocols
References
ab155585 has not yet been referenced specifically in any publications.
