Overview

  • Product name

  • Description

    Rabbit polyclonal to PAQR5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Sheep, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the C terminal of Human PAQR5

  • Positive control

    • Extracts from HUVEC cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/5000.

Target

  • Relevance

    PAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family.
  • Cellular localization

    Membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • FLJ20190 antibody
    • Membrane progestin receptor gamma antibody
    • mPR gamma antibody
    • MPRG antibody
    • PAQR 5 antibody
    • Progestin and adipoQ receptor family member V antibody
    see all

Images

  • All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • All lanes : Anti-PAQR5 antibody (ab79517) at 500 µg

    Lane 1 : HUVEC cell lysate (positive control)
    Lanes 2-4 : Non-pregnant ovine caruncle tissue lysate
    Lanes 5-7 : Day 30 of gestation ovine caruncle tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 52 kDa (possible non-specific binding)

    See Abreview

  • ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:

  • Tocchetti GN  et al. Biphasic modulation of cAMP levels by the contraceptive nomegestrol acetate. Impact on P-glycoprotein expression and activity in hepatic cells. Biochem Pharmacol 154:118-126 (2018). WB . Read more (PubMed: 29684377) »
  • Jackson AC  et al. Progesterone receptor signalling in retinal photoreceptor neuroprotection. J Neurochem 136:63-77 (2016). Read more (PubMed: 26447367) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Sheep Purified protein (caruncle tissue)
Specification
caruncle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 09 2014

Answer

Thank you for contacting Abcam. The immunogen peptide for ab75508 is is from *******and for ab79517, the immnuogen peptide is from ******, both peptides are from the human sequence. If there is anything else I can help you with, please let me know.

Read More
Application
Immunocytochemistry
Sample
Mouse Cell (Mouse ovary sections)
Specification
Mouse ovary sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Mr. Nicholas Bashour

Verified customer

Submitted Dec 09 2011

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