Product nameAnti-PAQR5 antibody
See all PAQR5 primary antibodies
DescriptionRabbit polyclonal to PAQR5
Tested applicationsSuitable for: ICC, WB, ELISAmore details
Species reactivityReacts with: Mouse, Sheep, Human
Predicted to work with: Rat
Synthetic peptide derived from the C terminal of Human PAQR5
- Extracts from HUVEC cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
RelevancePAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family.
Cellular localizationMembrane; Multi-pass membrane protein.
- FLJ20190 antibody
- Membrane progestin receptor gamma antibody
- mPR gamma antibody
All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
All lanes : Anti-PAQR5 antibody (ab79517) at 500 µg
Lane 1 : HUVEC cell lysate (positive control)
Lanes 2-4 : Non-pregnant ovine caruncle tissue lysate
Lanes 5-7 : Day 30 of gestation ovine caruncle tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible non-specific binding)
ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).
Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
This product has been referenced in:
- Tocchetti GN et al. Biphasic modulation of cAMP levels by the contraceptive nomegestrol acetate. Impact on P-glycoprotein expression and activity in hepatic cells. Biochem Pharmacol 154:118-126 (2018). WB . Read more (PubMed: 29684377) »
- Jackson AC et al. Progesterone receptor signalling in retinal photoreceptor neuroprotection. J Neurochem 136:63-77 (2016). Read more (PubMed: 26447367) »