Key features and details
- Mouse polyclonal to PAR-3/PARD3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAR-3/PARD3 antibody
See all PAR-3/PARD3 primary antibodies
DescriptionMouse polyclonal to PAR-3/PARD3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PAR-3/PARD3 aa 1-1340.
MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHR LEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQS PEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALIG LSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYR SLPRDTSNWSNQFQRDNARSSLSASHPMVGKWLEKQEQDEDGTEEDNSRV EPVGHADTGLEHIPNFSLDDMVKLVEVPNDGGPLGIHVVPFSARGGRTLG LLVKRLEKGGKAEHENLFRENDCIVRINDGDLRNRRFEQAQHMFRQAMRT PIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSVNSAGLH TVQRAPRLNHPPEQIDSHSRLPHSAHPSGKPPSAPASAPQNVFSTTVSSG YNTKKIGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAI QDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRSTKMEGTVSLLVFRQEDA FHPRELKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKEN HADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKTNQDAMETLR RSMSTEGNKRGMIQLIVARRISKCNELKSPGSPPGPELPIETALDDRERR ISHSLYSGIEGLDESPSRNAALSRIMGKYQLSPTVNMPQDDTVIIEDNRL PVLPPHLSDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVL AFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLGIADETKLNTV DDQKAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRPRII RGRGCNESFRAAIDKSYDKPAVDDDDEGMETLEEDTEESSRSGRESVSTA SDQPSHSLERQMNGNQEKGDKTDRKKDKTGKEKKKDRDKEKDKMKAKKGM LKGLGDMFRFGKHRKDDKIEKTGKIKIQESFTSEEERIRMKQEQERIQAK TREFRERQARERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQS PREGHMMDALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQQAKQ DEDVEDRRRTYSFEQPWPNARPATQSGRHSVSVEVQMQRQRQEERESSQQ AQRQYSSLPRQSRKNASSVSQDSWEQNYSPGEGFQSAKENPRYSSYQGSR NGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQPPSEGPSNYDSYKKV QDPSYAPPKGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS
Database link: Q8TEW0-11
- PAR-3/PARD3 transfected 293T cell line lysate.
Previously labelled as PARD3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 150 kDa.|
FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the PAR3 family.
Contains 3 PDZ (DHR) domains.
DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
modificationsPhosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
- Information by UniProt
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ab168106 has not yet been referenced specifically in any publications.