Overview

  • Product name

  • Description

    Mouse polyclonal to PAR-3/PARD3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PAR-3/PARD3 aa 1-1340.
    Sequence:

    MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHR LEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQS PEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALIG LSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYR SLPRDTSNWSNQFQRDNARSSLSASHPMVGKWLEKQEQDEDGTEEDNSRV EPVGHADTGLEHIPNFSLDDMVKLVEVPNDGGPLGIHVVPFSARGGRTLG LLVKRLEKGGKAEHENLFRENDCIVRINDGDLRNRRFEQAQHMFRQAMRT PIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSVNSAGLH TVQRAPRLNHPPEQIDSHSRLPHSAHPSGKPPSAPASAPQNVFSTTVSSG YNTKKIGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAI QDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRSTKMEGTVSLLVFRQEDA FHPRELKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKEN HADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKTNQDAMETLR RSMSTEGNKRGMIQLIVARRISKCNELKSPGSPPGPELPIETALDDRERR ISHSLYSGIEGLDESPSRNAALSRIMGKYQLSPTVNMPQDDTVIIEDNRL PVLPPHLSDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVL AFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLGIADETKLNTV DDQKAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRPRII RGRGCNESFRAAIDKSYDKPAVDDDDEGMETLEEDTEESSRSGRESVSTA SDQPSHSLERQMNGNQEKGDKTDRKKDKTGKEKKKDRDKEKDKMKAKKGM LKGLGDMFRFGKHRKDDKIEKTGKIKIQESFTSEEERIRMKQEQERIQAK TREFRERQARERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQS PREGHMMDALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQQAKQ DEDVEDRRRTYSFEQPWPNARPATQSGRHSVSVEVQMQRQRQEERESSQQ AQRQYSSLPRQSRKNASSVSQDSWEQNYSPGEGFQSAKENPRYSSYQGSR NGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQPPSEGPSNYDSYKKV QDPSYAPPKGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS


    Database link: Q8TEW0-11

  • Positive control

    • PAR-3/PARD3 transfected 293T cell line lysate.
  • General notes

    Previously labelled as PARD3

Properties

Applications

Our Abpromise guarantee covers the use of ab168106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 150 kDa.

Target

  • Function

    Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the PAR3 family.
    Contains 3 PDZ (DHR) domains.
  • Domain

    Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
    The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
  • Post-translational
    modifications

    Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
    Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
  • Cellular localization

    Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • agouti signaling protein antibody
    • ASIP antibody
    • Atypical PKC isotype specific interacting protein antibody
    • Atypical PKC isotype-specific-interacting protein antibody
    • Baz antibody
    • bazooka antibody
    • Coagulation factor II receptor-like 2 antibody
    • CTCL tumor antigen se2-5 antibody
    • F2rl2 antibody
    • PAR-3 antibody
    • par-3 family cell polarity regulator alpha antibody
    • par-3 family cell polarity regulator antibody
    • PAR3 alpha antibody
    • par3 antibody
    • PAR3-alpha antibody
    • PARD-3 antibody
    • Pard3 antibody
    • PARD3_HUMAN antibody
    • PARD3A antibody
    • Partitioning defective 3 homolog antibody
    • Partitioning-defective 3 homolog antibody
    • Partitioning-defective protein 3, C. elegans, homolog of antibody
    • SE2-5L16 antibody
    • SE2-5LT1 antibody
    • SE2-5T2 antibody
    • Thrombin receptor-like 2 antibody
    see all

Images

  • All lanes : Anti-PAR-3/PARD3 antibody (ab168106) at 1 µg/ml

    Lane 1 : PARD3 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 150 kDa

References

ab168106 has not yet been referenced specifically in any publications.

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