Key features and details
- Rabbit polyclonal to PAR-3/PARD3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PAR-3/PARD3 antibody
See all PAR-3/PARD3 primary antibodies
DescriptionRabbit polyclonal to PAR-3/PARD3
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Chimpanzee
Previously labelled as PARD3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 0.5 µg/ml.|
FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the PAR3 family.
Contains 3 PDZ (DHR) domains.
DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
modificationsPhosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
- Information by UniProt
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IHC image of PAR-3/PARD3 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64646, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab64646 stained A549 cells. The cells were 100% methanol fixed for 5 minutes at-20°C* and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64646 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-PAR-3/PARD3 antibody (ab64646) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Thymus (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 150 kDa
Additional bands at: 40 kDa (possible non-specific binding), 45 kDa (possible non-specific binding), 54 kDa (possible non-specific binding), 68 kDa (possible non-specific binding)
Exposure time: 4 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk before being incubated with ab64646 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64646 has been referenced in 7 publications.
- Zhang X et al. MicroRNA 483-3p targets Pard3 to potentiate TGF-ß1-induced cell migration, invasion, and epithelial-mesenchymal transition in anaplastic thyroid cancer cells. Oncogene 38:699-715 (2019). PubMed: 30171257
- Li J et al. Pard3 suppresses glioma invasion by regulating RhoA through atypical protein kinase C/NF-?B signaling. Cancer Med 8:2288-2302 (2019). PubMed: 30848088
- Stypulkowski E et al. The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division. Sci Signal 11:N/A (2018). PubMed: 29295957
- Michel L et al. Study of gene expression alteration in male androgenetic alopecia: evidence of predominant molecular signalling pathways. Br J Dermatol 177:1322-1336 (2017). PubMed: 28403520
- Song T et al. Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3? protein. Oncotarget 7:64260-64273 (2016). PubMed: 27588399
- Tong S et al. 14-3-3? promotes lung cancer cell invasion by increasing the Snail protein expression through atypical protein kinase C (aPKC)/NF-?B signaling. Exp Cell Res 348:1-9 (2016). PubMed: 27554601
- Nakamura H et al. Expression of Par3 polarity protein correlates with poor prognosis in ovarian cancer. BMC Cancer 16:897 (2016). ICC/IF ; Human . PubMed: 27855669