This antibody produced a pretty cytoplasmic and membrane bound staining in cortical neurons, when diluted 1:100. Unfortunately, the staining is not very contrasted.
The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Dr. Sophie Pezet
Submitted Jul 29 2011
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