Immunohistochemistry free floating abreview for Anti-PAR-3/PARD3 antibody

Good
Abreviews
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
This antibody produced a pretty cytoplasmic and membrane bound staining in cortical neurons, when diluted 1:100. Unfortunately, the staining is not very contrasted. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Dr. Sophie Pezet

Verified customer

Submitted Jul 29 2011

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