Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PAR-3/PARD3 antibody [EP310Y]
See all PAR-3/PARD3 primary antibodies -
Description
Rabbit monoclonal [EP310Y] to PAR-3/PARD3 -
Host species
Rabbit -
Specificity
This antibody is predicted to detect isoforms 2-7 based on sequence analysis. -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PAR-3/PARD3 aa 1300-1400 (C terminal). The exact sequence is proprietary.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as PARD3
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP310Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab40769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the PAR3 family.
Contains 3 PDZ (DHR) domains. -
Domain
Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids. -
Post-translational
modificationsPhosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity. -
Cellular localization
Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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Database links
- Entrez Gene: 56288 Human
- Omim: 606745 Human
- SwissProt: Q8TEW0 Human
- Unigene: 131489 Human
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Alternative names
- agouti signaling protein antibody
- ASIP antibody
- Atypical PKC isotype specific interacting protein antibody
see all
Images
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![Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)](https://www.abcam.com/ps/products/40/ab40769/Images/ab40769-53896-anti-par-3-pard3-antibody-ep310y-western-blot.jpg)
Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)All lanes : Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769) at 1/500 dilution
Lane 1 : LNCap
Lane 2 : HeLa
Lane 3 : A431
Lane 4 : MCF-7
Lysates/proteins at 10 µg per lane.
Predicted band size: 150 kDa
Observed band size: 150 kDa
Datasheets and documents
References
This product has been referenced in:
- Cho JH et al. Identification of the novel substrates for caspase-6 in apoptosis using proteomic approaches. BMB Rep 46:588-93 (2013). Read more (PubMed: 24195789) »
