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Signal Transduction Adapters Transmembrane
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RabMAb

Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)

  • Datasheet
Submit a review Q&A (1)References (1)

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Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)

    Key features and details

    • Rabbit monoclonal [EP310Y] to PAR-3/PARD3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-PAR-3/PARD3 antibody [EP310Y]
      See all PAR-3/PARD3 primary antibodies
    • Description

      Rabbit monoclonal [EP310Y] to PAR-3/PARD3
    • Host species

      Rabbit
    • Specificity

      This antibody is predicted to detect isoforms 2-7 based on sequence analysis.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human PAR-3/PARD3 aa 1300-1400 (C terminal). The exact sequence is proprietary.

    • General notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Previously labelled as PARD3

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.20
      Preservative: 0.05% Sodium azide
      Constituents: 99% Tissue culture supernatant, 0.1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      EP310Y
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Adapters
      • Transmembrane
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Tight Junctions

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab40769 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
    Notes
    WB
    1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
  • Application notes
    Is unsuitable for ICC,IHC or IP.
  • Target

    • Function

      Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the PAR3 family.
      Contains 3 PDZ (DHR) domains.
    • Domain

      Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
      The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
    • Post-translational
      modifications

      Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
      Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
    • Cellular localization

      Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Target information above from: UniProt accession Q8TEW0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 56288 Human
      • Omim: 606745 Human
      • SwissProt: Q8TEW0 Human
      • Unigene: 131489 Human
      • Alternative names

        • agouti signaling protein antibody
        • ASIP antibody
        • Atypical PKC isotype specific interacting protein antibody
        • Atypical PKC isotype-specific-interacting protein antibody
        • Baz antibody
        • bazooka antibody
        • Coagulation factor II receptor-like 2 antibody
        • CTCL tumor antigen se2-5 antibody
        • F2rl2 antibody
        • PAR-3 antibody
        • par-3 family cell polarity regulator alpha antibody
        • par-3 family cell polarity regulator antibody
        • PAR3 alpha antibody
        • par3 antibody
        • PAR3-alpha antibody
        • PARD-3 antibody
        • Pard3 antibody
        • PARD3_HUMAN antibody
        • PARD3A antibody
        • Partitioning defective 3 homolog antibody
        • Partitioning-defective 3 homolog antibody
        • Partitioning-defective protein 3, C. elegans, homolog of antibody
        • SE2-5L16 antibody
        • SE2-5LT1 antibody
        • SE2-5T2 antibody
        • Thrombin receptor-like 2 antibody
        see all

      Images

      • Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)
        Western blot - Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769)
        All lanes : Anti-PAR-3/PARD3 antibody [EP310Y] (ab40769) at 1/500 dilution

        Lane 1 : LNCap
        Lane 2 : HeLa
        Lane 3 : A431
        Lane 4 : MCF-7

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 150 kDa
        Observed band size: 150 kDa

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab40769? Please let us know so that we can cite the reference in this datasheet.

      ab40769 has been referenced in 1 publication.

      • Cho JH  et al. Identification of the novel substrates for caspase-6 in apoptosis using proteomic approaches. BMB Rep 46:588-93 (2013). PubMed: 24195789

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Will this antibody react with other PAR's as well such as Par4 or Par1?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2006

      Answer

      Based on the sequence alignment, I could not find any information which suggested this antibody would detect PAR4 or 1. I hope this information helps and please don't hesitate to contact us for more advice or information in the future.

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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