Overview

  • Product name

    Anti-PAR1/Thrombin Receptor antibody
    See all PAR1/Thrombin Receptor primary antibodies
  • Description

    Rabbit polyclonal to PAR1/Thrombin Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PAR1/Thrombin Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab138715)

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma. This antibody gave a positive signal in WB within Human Platelet tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab117749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
  • Tissue specificity

    Platelets and vascular endothelial cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
    Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CF2R antibody
    • Coagulation factor II (thrombin) receptor antibody
    • Coagulation factor II receptor antibody
    • F2R antibody
    • HTR antibody
    • PAR 1 antibody
    • PAR-1 antibody
    • PAR1 antibody
    • PAR1_HUMAN antibody
    • Protease activated receptor 1 antibody
    • Proteinase activated receptor 1 antibody
    • Proteinase-activated receptor 1 antibody
    • Thrombin receptor antibody
    • TR antibody
    see all

Images

  • Anti-PAR1/Thrombin Receptor antibody (ab117749) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 116 kDa, 33 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    PAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • IHC image of PAR1/Thrombin Receptor staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117749, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab117749 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab117749.
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