Anti-PAR1/Thrombin Receptor antibody (ab183083)
Key features and details
- Rabbit polyclonal to PAR1/Thrombin Receptor
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAR1/Thrombin Receptor antibody
See all PAR1/Thrombin Receptor primary antibodies -
Description
Rabbit polyclonal to PAR1/Thrombin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon
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Immunogen
Synthetic peptide within Human PAR1/Thrombin Receptor aa 50-150 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001983.
Database link: P25116 -
Positive control
- WB: K562 cell lysate; Human lymphoma and fetal kidney tissue lysates; IHC-P: Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/40 - 1/200.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
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| Notes |
|---|
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IHC-P
1/40 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
Target
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Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P56488 Baboon
- SwissProt: P25116 Human
- Unigene: 482562 Human
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Alternative names
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab183083)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PAR1/Thrombin Receptor with ab183083 at 1/40 dilution. The image on the right is treated with the immunizing peptide.
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Western blot - Anti-PAR1/Thrombin Receptor antibody (ab183083)All lanes : Anti-PAR1/Thrombin Receptor antibody (ab183083) at 1/650 dilution
Lane 1 : K562 cell lysate
Lane 2 : Human lymphoma tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab183083 has been referenced in 2 publications.
- Yi X et al. Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA-Seq. J Cell Physiol N/A:N/A (2019). PubMed: 30989650
- Ju XN et al. Baicalin protects against thrombin induced cell injury in SH-SY5Y cells. Int J Clin Exp Pathol 8:14021-7 (2015). WB ; Human . PubMed: 26823714