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Anti-PAR1/Thrombin Receptor antibody (ab183083)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab183083)
  • Western blot - Anti-PAR1/Thrombin Receptor antibody (ab183083)

Key features and details

  • Rabbit polyclonal to PAR1/Thrombin Receptor
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human PAR1/Thrombin Receptor protein (ab114346)
Primary
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Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PAR1/Thrombin Receptor antibody
    See all PAR1/Thrombin Receptor primary antibodies
  • Description

    Rabbit polyclonal to PAR1/Thrombin Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Baboon
  • Immunogen

    Synthetic peptide within Human PAR1/Thrombin Receptor aa 50-150 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001983.
    Database link: P25116

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: K562 cell lysate; Human lymphoma and fetal kidney tissue lysates; IHC-P: Human colon cancer tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human PAR1/Thrombin Receptor protein (ab114346)
  • Related Products

    • Recombinant Human PAR1/Thrombin Receptor protein (ab114346)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/40 - 1/200.
WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
Notes
IHC-P
1/40 - 1/200.
WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa.

Target

  • Function

    High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
  • Tissue specificity

    Platelets and vascular endothelial cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
    Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P25116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2149 Human
    • Omim: 187930 Human
    • SwissProt: P56488 Baboon
    • SwissProt: P25116 Human
    • Unigene: 482562 Human
    • Alternative names

      • CF2R antibody
      • Coagulation factor II (thrombin) receptor antibody
      • Coagulation factor II receptor antibody
      • F2R antibody
      • HTR antibody
      • PAR 1 antibody
      • PAR-1 antibody
      • PAR1 antibody
      • PAR1_HUMAN antibody
      • Protease activated receptor 1 antibody
      • Proteinase activated receptor 1 antibody
      • Proteinase-activated receptor 1 antibody
      • Thrombin receptor antibody
      • TR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab183083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab183083)

      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PAR1/Thrombin Receptor with ab183083 at 1/40 dilution. The image on the right is treated with the immunizing peptide.

    • Western blot - Anti-PAR1/Thrombin Receptor antibody (ab183083)
      Western blot - Anti-PAR1/Thrombin Receptor antibody (ab183083)
      All lanes : Anti-PAR1/Thrombin Receptor antibody (ab183083) at 1/650 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Human lymphoma tissue lysate
      Lane 3 : Human fetal kidney tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa


      Exposure time: 2 minutes

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab183083? Please let us know so that we can cite the reference in this datasheet.

    ab183083 has been referenced in 2 publications.

    • Yi X  et al. Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA-Seq. J Cell Physiol N/A:N/A (2019). PubMed: 30989650
    • Ju XN  et al. Baicalin protects against thrombin induced cell injury in SH-SY5Y cells. Int J Clin Exp Pathol 8:14021-7 (2015). WB ; Human . PubMed: 26823714

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunoprecipitation abreview for Anti-PAR1/Thrombin Receptor antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Megakaryoblast)
    Total protein in input
    90 µg
    Immuno-precipitation step
    Protein A
    Specification
    Megakaryoblast
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 21 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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