Anti-PAR1/Thrombin Receptor antibody (ab183083)
Overview
-
Product name
Anti-PAR1/Thrombin Receptor antibody
See all PAR1/Thrombin Receptor primary antibodies -
Description
Rabbit polyclonal to PAR1/Thrombin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon
-
Immunogen
Synthetic peptide within Human PAR1/Thrombin Receptor aa 56-70 (N terminal). The exact sequence is proprietary. NP_001983.
Sequence:WEDEEKNESGLTEYR
Database link: P25116 -
Positive control
- WB: K562 cell lysate; Human lymphoma and fetal kidney tissue lysates; IHC-P: Human colon cancer tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab183083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/40 - 1/200. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
Target
-
Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P56488 Baboon
- SwissProt: P25116 Human
- Unigene: 482562 Human
-
Alternative names
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab183083)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PAR1/Thrombin Receptor with ab183083 at 1/40 dilution. The image on the right is treated with the immunizing peptide.
-
Western blot - Anti-PAR1/Thrombin Receptor antibody (ab183083)All lanes : Anti-PAR1/Thrombin Receptor antibody (ab183083) at 1/650 dilution
Lane 1 : K562 cell lysate
Lane 2 : Human lymphoma tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 2 minutes
Protocols
Datasheets and documents
References
ab183083 has been referenced in 2 publications.
- Yi X et al. Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA-Seq. J Cell Physiol N/A:N/A (2019). PubMed: 30989650
- Ju XN et al. Baicalin protects against thrombin induced cell injury in SH-SY5Y cells. Int J Clin Exp Pathol 8:14021-7 (2015). WB ; Human . PubMed: 26823714
