Anti-PAR1/Thrombin Receptor antibody (ab32611)
Key features and details
- Rabbit polyclonal to PAR1/Thrombin Receptor
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAR1/Thrombin Receptor antibody
See all PAR1/Thrombin Receptor primary antibodies -
Description
Rabbit polyclonal to PAR1/Thrombin Receptor -
Host species
Rabbit -
Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab233741.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon -
Immunogen
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Positive control
- KNRK cells, Pancreatic tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P56488 Baboon
- SwissProt: P25116 Human
- Unigene: 482562 Human
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Alternative names
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
Images
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Anti-PAR1/Thrombin Receptor antibody (ab32611) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesPAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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This image shows human pancreas stained with ab32611.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab32611 has been referenced in 13 publications.
- Guan Y et al. Protease-Activated Receptor 1 Contributes to Microcirculation Failure and Tubular Damage in Renal Ischemia-Reperfusion Injury in Mice. Biomed Res Int 2021:6665714 (2021). PubMed: 33681367
- Dekker NAM et al. Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental cardiopulmonary bypass. Intensive Care Med Exp 9:30 (2021). PubMed: 34169407
- Gerasimov A et al. Markers for neural degeneration and regeneration: novel highly sensitive methods for the measurement of thrombin and activated protein C in human cerebrospinal fluid. Neural Regen Res 16:2086-2092 (2021). PubMed: 33642398
- Perry EA et al. Tetracyclines promote survival and fitness in mitochondrial disease models. Nat Metab 3:33-42 (2021). PubMed: 33462515
- Shavit-Stein E et al. Brain Protease Activated Receptor 1 Pathway: A Therapeutic Target in the Superoxide Dismutase 1 (SOD1) Mouse Model of Amyotrophic Lateral Sclerosis. Int J Mol Sci 21:N/A (2020). PubMed: 32408605