Anti-PAR2 antibody [SAM11] (ab184673)
Key features and details
- Mouse monoclonal [SAM11] to PAR2
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PAR2 antibody [SAM11]
See all PAR2 primary antibodies -
Description
Mouse monoclonal [SAM11] to PAR2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length protein corresponding to Human PAR2.
Database link: P55085 -
Epitope
ab184673 was mapped to amino acids 37 - 50. -
Positive control
- Human heart tissue; Mouse 3T3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SAM11 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 44 kDa.
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IHC-P | (1) |
1/100.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Predicted molecular weight: 44 kDa. |
IHC-P
1/100. |
Target
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Function
Receptor for trypsin and trypsin-like enzymes coupled to G proteins that stimulate phosphoinositide hydrolysis. May have a role in the regulation of vascular tone. -
Tissue specificity
Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2150 Human
- Entrez Gene: 14063 Mouse
- Omim: 600933 Human
- SwissProt: P55085 Human
- SwissProt: P55086 Mouse
- Unigene: 154299 Human
- Unigene: 1614 Mouse
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Alternative names
- Coagulation factor II receptor like 1 antibody
- Coagulation factor II receptor-like 1 antibody
- Coagulation factor II thrombin receptor like 1 antibody
see all
Images
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All lanes : Anti-PAR2 antibody [SAM11] (ab184673) at 1/1000 dilution
Lane 1 : Mouse 3T3 cell lysate at 10 µg
Lane 2 : Mouse 3T3 cell lysate at 20 µg
Lane 3 : BSA control
Predicted band size: 44 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling PAR2 with ab184673 at 10μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab184673 has been referenced in 8 publications.
- Francischetti IMB et al. Neutrophil and Eosinophil Extracellular Traps in Hodgkin Lymphoma. Hemasphere 5:e633 (2021). PubMed: 34485830
- Pierre O et al. Calcium Increase and Substance P Release Induced by the Neurotoxin Brevetoxin-1 in Sensory Neurons: Involvement of PAR2 Activation through Both Cathepsin S and Canonical Signaling. Cells 9:N/A (2020). PubMed: 33348659
- Wypasek E et al. Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves. Vascul Pharmacol 130:106679 (2020). PubMed: 32387621
- Hoffman S et al. Fecal Proteases from Pouchitis Patients Activate Protease Activating Receptor-2 to Disrupt the Epithelial Barrier. J Crohns Colitis N/A:N/A (2019). PubMed: 31056700
- Li TZ et al. Protease-activated receptor-2 regulates glial scar formation via JNK signaling. Physiol Res N/A:N/A (2019). PubMed: 30628825
- Lianto P et al. Quail egg homogenate alleviates food allergy induced eosinophilic esophagitis like disease through modulating PAR-2 transduction pathway in peanut sensitized mice. Sci Rep 8:1049 (2018). PubMed: 29348584
- DuMont AL & Cianciotto NP Stenotrophomonas maltophilia Serine Protease StmPr1 Induces Matrilysis, Anoikis, and Protease-Activated Receptor 2 Activation in Human Lung Epithelial Cells. Infect Immun 85:N/A (2017). PubMed: 28893914
- Villari A et al. Apixaban Enhances Vasodilatation Mediated by Protease-Activated Receptor 2 in Isolated Rat Arteries. Front Pharmacol 8:480 (2017). PubMed: 28769809