Recombinant Anti-PAR4 antibody [EPR3992] (ab109174)
![Western blot - Anti-PAR4 antibody [EPR3992] (ab109174)](https://www.abcam.com/ps/products/109/ab109174/Images/ab109174-121741-anti-par4-antibody-epr3992-western-blot.jpg "Western blot - Anti-PAR4 antibody [EPR3992] (ab109174)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3992] to PAR4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PAR4 antibody [EPR3992]
See all PAR4 primary antibodies -
Description
Rabbit monoclonal [EPR3992] to PAR4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PAR4 aa 300-400. The exact sequence is proprietary.
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Positive control
- LnCaP, HeLa, Jurkat, A549 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3992 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. -
Tissue specificity
Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers. -
Domain
The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells. -
Post-translational
modificationsPreferentially phosphorylated at the Thr-163 by PKC in cancer cells. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 5074 Human
- Entrez Gene: 114774 Mouse
- Entrez Gene: 64513 Rat
- Omim: 601936 Human
- SwissProt: Q96IZ0 Human
- SwissProt: Q925B0 Mouse
- SwissProt: Q62627 Rat
- Unigene: 643130 Human
see all -
Alternative names
- 2310001G03Rik antibody
- PAR 4 antibody
- PAR-4 antibody
see all
Images
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Western blot - Anti-PAR4 antibody [EPR3992] (ab109174)All lanes : Anti-PAR4 antibody [EPR3992] (ab109174) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
![Anti-PAR4 antibody [EPR3992] (ab109174)](https://www.abcam.com/ps/products/109/ab109174/Images/ab109174-2-benefits-of-recombinant-antibodies.png)
Anti-PAR4 antibody [EPR3992] (ab109174)
Datasheets and documents
References (1)
ab109174 has been referenced in 1 publication.
- Chen X et al. Fbxo45-mediated degradation of the tumor-suppressor Par-4 regulates cancer cell survival. Cell Death Differ 21:1535-45 (2014). PubMed: 24992930
