Overview

  • Product name

  • Description

    Rabbit polyclonal to PAR6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PAR6 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab49775)

  • Positive control

    • This antibody gave a positive signal using a PARD6A partial Human Recombinant Tagged Protein

Properties

Applications

Our Abpromise guarantee covers the use of ab49776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
  • Tissue specificity

    Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
  • Sequence similarities

    Belongs to the PAR6 family.
    Contains 1 OPR domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 pseudo-CRIB domain.
  • Domain

    The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
    The OPR domain mediates interactions with MAP2K5.
    The PDZ domain mediates the interaction with CRB3.
  • Cellular localization

    Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0710008C04Rik antibody
    • 2610010A15Rik antibody
    • Par 6 partitioning defective 6 C elegans homolog alpha antibody
    • Par 6 partitioning defective 6 homolog alpha antibody
    • Par 6 partitioning defective 6 homolog alpha C elegans antibody
    • PAR 6A antibody
    • PAR-6 alpha antibody
    • PAR-6 antibody
    • par-6 family cell polarity regulator alpha antibody
    • PAR-6A antibody
    • PAR6 antibody
    • PAR6A_HUMAN antibody
    • PAR6alpha antibody
    • PAR6C antibody
    • Pard6a antibody
    • Partitioning defective 6 homolog alpha antibody
    • partitioning-defective protein 6 antibody
    • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
    • Tax interacting protein 40 antibody
    • Tax interaction protein 40 antibody
    • TAX40 antibody
    • TIP 40 antibody
    • TIP-40 antibody
    • TIP40 antibody
    see all

Images

  • Anti-PAR6 antibody (ab49776) at 1 µg/ml + PARD6A partial Human Recombinant Tagged Protein at 0.2 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • ICC/IF image of ab49776 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49776, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells.

References

This product has been referenced in:

  • Bai X  et al. A de novo Mutation in the MTUS1 Gene Decreases the Risk of Non-compaction of Ventricular Myocardium via the Rac1/Cdc42 Pathway. Front Pediatr 7:247 (2019). Read more (PubMed: 31338350) »
  • Kusne Y  et al. Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFa in glioblastoma. Sci Signal 7:ra75 (2014). Read more (PubMed: 25118327) »
See all 5 Publications for this product

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