Overview

  • Product name
  • Description
    Goat polyclonal to PAR6
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Peptide with sequence

    GSRIRGDGSGFSL

    , from C Terminus of the protein sequence according to NP_058644

  • Positive control
    • Human Brain and Pancreas extracts.
  • General notes
    GenBank Accession Number – NP_058644.

Properties

Applications

Our Abpromise guarantee covers the use of ab6022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:8,000.
    IHC-P: Use at a concentration of 5 - 10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
    • Tissue specificity
      Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
    • Sequence similarities
      Belongs to the PAR6 family.
      Contains 1 OPR domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 pseudo-CRIB domain.
    • Domain
      The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
      The OPR domain mediates interactions with MAP2K5.
      The PDZ domain mediates the interaction with CRB3.
    • Cellular localization
      Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0710008C04Rik antibody
      • 2610010A15Rik antibody
      • Par 6 partitioning defective 6 C elegans homolog alpha antibody
      • Par 6 partitioning defective 6 homolog alpha antibody
      • Par 6 partitioning defective 6 homolog alpha C elegans antibody
      • PAR 6A antibody
      • PAR-6 alpha antibody
      • PAR-6 antibody
      • par-6 family cell polarity regulator alpha antibody
      • PAR-6A antibody
      • PAR6 antibody
      • PAR6A_HUMAN antibody
      • PAR6alpha antibody
      • PAR6C antibody
      • Pard6a antibody
      • Partitioning defective 6 homolog alpha antibody
      • partitioning-defective protein 6 antibody
      • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
      • Tax interacting protein 40 antibody
      • Tax interaction protein 40 antibody
      • TAX40 antibody
      • TIP 40 antibody
      • TIP-40 antibody
      • TIP40 antibody
      see all

    Images

    • Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

      Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.

    • ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your inquiry.

    Unfortunately, we only have one antibody in our catalog at the moment that is tested to work with mouse. The immunogen of does not show a high homology with mouse but the antibody was used successfully in the following publication:

    PubMed: 20643355, Figure 7 IHC.

    The antibody most likely binds to the last coupleof amino acids in the immunogen. This would also mean that all three isoform should be recognized.

    But this has not been experimentally tested and we do not know yetthat this antibody is good for mouse under all circumstances and applications in all mouse samples.

    I am sorry I did not have positive and straight forward information for you on this occasion and hope this information is nevertheless helpful to you.

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    Answer

    Comme convenu, la commande numéro ****** (une unité gratuite de ab6022) a été expédiée aujourd'hui. Vous devriez recevoir le produit demain. J'espère que l'anti-PAR6 ab6022 fonctionnera chez la souris. Nous serions ravis si vous pouviez nous envoyer une Abreview avec votre feedback sur l'utilisation de ab6022 chez la souris. Le lien pour soumettre cette Abreview est : https://www.abcam.com/PAR6-antibody-ab6022-reviews.html N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Answer

    Thank you for contacting us. I have heard of people using anti-vimentin clone V9, to detect human tissue implanted in mice. However, this is a mouse monoclonal and our version, ab8069 is unconjugated. We do not have a version conjugated to a fluorochrome, enzyme, or other detector. An anti-mouse IgG secondary may give some staining of endogenous IgG, as you are probably aware. For detecting mouse tissue without detecting human, consider ab15630, with is also available biotinylated as ab15631 and FITC-conjugated as ab15638: https://www.abcam.com/index.html?datasheet=15630 The ab15630 and ab15631 have been shown to work on frozen tissue, but will not work on paraffin-embedded tissue. Ab15638 has only been tested for flow cytometry so we canot guarantee it will work for IHC. Antibody ab6022 has not been tested on mouse tissue. An alignment of the immunogenic peptide with the mouse protein shows that it is 69% identical; it is difficult to predict from this if the antibody will or will not cross-react with the mouse protein. The alignment can be found at this address for the next 24 hours: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070510-00025988&poll=yes I hope this helps; please do not hesitate to contact us if you have any questions.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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